Does the maternal vaginal microbiota play a role in seeding the microbiota of neonatal gut and nose?
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- O. Sakwinska
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- F. Foata
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- B. Berger
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- H. Brüssow
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- S. Combremont
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- A. Mercenier
- Nestlé Research Center, Vers-Chez-Les-Blanc, 1000 Lausanne, Switzerland.
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- S. Dogra
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
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- S.-E. Soh
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
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- J.C.K. Yen
- Department of Reproductive Medicine, KK Women’s and Children’s Hospital, 100 Bukit Timah Road, 229899 Singapore.
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- G.Y.S. Heong
- Department of Maternal Fetal Medicine, KK Women’s and Children’s Hospital, 100 Bukit Timah Road, 229899 Singapore.
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- Y.S. Lee
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
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- F. Yap
- Department of Paediatric Endocrinology, KK Women’s and Children’s Hospital, 100 Bukit Timah Road, 229899 Singapore.
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- M.J. Meaney
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
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- Y.-S. Chong
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
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- K.M. Godfrey
- MRC Lifecourse Epidemiology Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust, Tremona Road, Southampton SO16 6YD, United Kingdom.
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- J.D. Holbrook
- Singapore Institute for Clinical Sciences (SICS), Agency for Science and Technology Research (A*STAR), 30 Medical Drive, 117609 Singapore.
抄録
<jats:p>The acquisition and early maturation of infant microbiota is not well understood despite its likely influence on later health. We investigated the contribution of the maternal microbiota to the microbiota of infant gut and nose in the context of mode of delivery and feeding. Using 16S rRNA sequencing and specific qPCR, we profiled microbiota of 42 mother-infant pairs from the GUSTO birth cohort, at body sites including maternal vagina, rectum and skin; and infant stool and nose. In our study, overlap between maternal vaginal microbiota and infant faecal microbiota was minimal, while the similarity between maternal rectal microbiota and infant microbiota was more pronounced. However, an infant’s nasal and gut microbiota were no more similar to that of its own mother, than to that of unrelated mothers. These findings were independent of delivery mode. We conclude that the transfer of maternal vaginal microbes play a minor role in seeding infant stool microbiota. Transfer of maternal rectal microbiota could play a larger role in seeding infant stool microbiota, but approaches other than the generally used analyses of community similarity measures are likely to be needed to quantify bacterial transmission. We confirmed the clear difference between microbiota of infants born by Caesarean section compared to vaginally delivered infants and the impact of feeding mode on infant gut microbiota. Only vaginally delivered, fully breastfed infants had gut microbiota dominated by <jats:italic>Bifidobacteria.</jats:italic> Our data suggest that reduced transfer of maternal vaginal microbial is not the main mechanism underlying the differential infant microbiota composition associated with Caesarean delivery. The sources of a large proportion of infant microbiota could not be identified in maternal microbiota, and the sources of seeding of infant gut and nasal microbiota remain to be elucidated.</jats:p>
収録刊行物
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- Beneficial Microbes
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Beneficial Microbes 8 (5), 763-778, 2017-10-13
Brill