G-tail telomere HPA: simple measurement of human single-stranded telomeric overhangs

Hidetoshi Tahara, Mayuko Kusunoki, Yusuke Yamanaka, Shusaku Matsumura, Toshinori Ide

doi:10.1038/nmeth797

Description

Accurate measurement of telomeric 3'-overhang (G-tail) lengths is essential for investigation of the biological effects of telomere dysfunction. G-tail telomere hybridization protection assay (Gt-telomere HPA) has the advantages of being simple to perform, accurate and highly sensitive for G tails as short as 20 nucleotides. Furthermore, Gt-telomere HPA is specific and quantitative for human G tails, and can be used to assay cell lysates as well as genomic DNA.

Journal

Nature Methods

Nature Methods 2 (11), 829-831, 2005-10-21

Springer Science and Business Media LLC

Keywords

Details 詳細情報について

CRID: 1360574095041720832

NII Article ID: 80017743878

DOI: 10.1038/nmeth797

ISSN: 15487105; 15487091

PubMed: 16278652

Web Site: http://www.nature.com/articles/nmeth797.pdf; http://www.nature.com/articles/nmeth797

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