High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus

  • Natalie H. Chapman
    Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicstershire LE12 5RD, United Kingdom (N.H.C., N.G., R.S., G.S., G.B.S.)
  • Julien Bonnet
    Syngenta Seeds Limited, 31790 Saint Sauveur, France (J.B., L.G.)
  • Laurent Grivet
    Syngenta Seeds Limited, 31790 Saint Sauveur, France (J.B., L.G.)
  • James Lynn
    Applied Statistical Solutions, Bishops Tachbrook, Leamington Spa CV33 9RJ, United Kingdom (J.L.)
  • Neil Graham
    Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicstershire LE12 5RD, United Kingdom (N.H.C., N.G., R.S., G.S., G.B.S.)
  • Rebecca Smith
    Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicstershire LE12 5RD, United Kingdom (N.H.C., N.G., R.S., G.S., G.B.S.)
  • Guiping Sun
    Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicstershire LE12 5RD, United Kingdom (N.H.C., N.G., R.S., G.S., G.B.S.)
  • Peter G. Walley
    School of Life Sciences, University of Warwick, Wellesbourne Campus, Warwick CV35 9EF, United Kingdom (P.G.W.)
  • Mervin Poole
    Campden BRI, Chipping Campden, Gloucestershire GL55 6LD, United Kingdom (M.P.)
  • Mathilde Causse
    Institut National de la Recherche Agronomique, UR1052, Unité de Génétique et Amélioration des Fruits et Légumes, Domaine Saint-Maurice, 84143 Montfavet cedex, France (M.C.)
  • Graham J. King
    Southern Cross Plant Science, Southern Cross University, Lismore, New South Wales 2480, Australia (G.J.K.); and
  • Charles Baxter
    Syngenta Seeds Limited, Jealott’s Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom (C.B.)
  • Graham B. Seymour
    Plant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicstershire LE12 5RD, United Kingdom (N.H.C., N.G., R.S., G.S., G.B.S.)

抄録

<jats:title>Abstract</jats:title> <jats:p>Fruit firmness in tomato (Solanum lycopersicum) is determined by a number of factors including cell wall structure, turgor, and cuticle properties. Firmness is a complex polygenic trait involving the coregulation of many genes and has proved especially challenging to unravel. In this study, a quantitative trait locus (QTL) for fruit firmness was mapped to tomato chromosome 2 using the Zamir Solanum pennellii interspecific introgression lines (ILs) and fine-mapped in a population consisting of 7,500 F2 and F3 lines from IL 2-3 and IL 2-4. This firmness QTL contained five distinct subpeaks, Firs.p.QTL2.1 to Firs.p.QTL2.5, and an effect on a distal region of IL 2-4 that was nonoverlapping with IL 2-3. All these effects were located within an 8.6-Mb region. Using genetic markers, each subpeak within this combinatorial locus was mapped to a physical location within the genome, and an ethylene response factor (ERF) underlying Firs.p.QTL2.2 and a region containing three pectin methylesterase (PME) genes underlying Firs.p.QTL2.5 were nominated as QTL candidate genes. Statistical models used to explain the observed variability between lines indicated that these candidates and the nonoverlapping portion of IL 2-4 were sufficient to account for the majority of the fruit firmness effects. Quantitative reverse transcription-polymerase chain reaction was used to quantify the expression of each candidate gene. ERF showed increased expression associated with soft fruit texture in the mapping population. In contrast, PME expression was tightly linked with firm fruit texture. Analysis of a range of recombinant lines revealed evidence for an epistatic interaction that was associated with this combinatorial locus.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 159 (4), 1644-1657, 2012-06-08

    Oxford University Press (OUP)

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