Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory
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- P Kirschner
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- B Springer
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- U Vogel
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- A Meier
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- A Wrede
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- M Kiekenbeck
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- F C Bange
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
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- E C Böttger
- Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
書誌事項
- 公開日
- 1993-11
- 権利情報
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- https://journals.asm.org/non-commercial-tdm-license
- DOI
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- 10.1128/jcm.31.11.2882-2889.1993
- 公開者
- American Society for Microbiology
この論文をさがす
説明
<jats:p>Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria easily allowed rapid and correct identification. The results of this study demonstrate that polymerase chain reaction-mediated direct sequence determination can be used as a rapid and reliable method for the identification of mycobacteria in the clinical laboratory. In addition, the prompt recognition of previously undescribed species is now feasible.</jats:p>
収録刊行物
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- Journal of Clinical Microbiology
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Journal of Clinical Microbiology 31 (11), 2882-2889, 1993-11
American Society for Microbiology