Single-particle reconstruction of biological macromolecules in electron microscopy – 30 years
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説明
<jats:title>Abstract</jats:title><jats:p>This essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.</jats:p>
収録刊行物
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- Quarterly Reviews of Biophysics
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Quarterly Reviews of Biophysics 42 (3), 139-158, 2009-08
Cambridge University Press (CUP)
