CRYPTOCHROME-mediated phototransduction by modulation of the potassium ion channel β-subunit redox sensor
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- Keri J. Fogle
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Lisa S. Baik
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Jerry H. Houl
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Tri T. Tran
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Logan Roberts
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Nicole A. Dahm
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
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- Yu Cao
- Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
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- Ming Zhou
- Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030
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- Todd C. Holmes
- Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697; and
説明
<jats:title>Significance</jats:title> <jats:p> CRYPTOCHROMES (CRYs) are blue light photoreceptors that mediate phototransduction in brain arousal neurons, as well as circadian light entrainment in <jats:italic>Drosophila</jats:italic> fruit flies. We describe how light-activated <jats:italic>Drosophila</jats:italic> CRY couples to membrane depolarization and increased action potential firing rate in large ventral lateral arousal neurons. Pharmacological treatments that specifically disrupt the CRY redox-sensitive flavin chromophore or block voltage-gated K <jats:sup>+</jats:sup> channels abolish the light response. Correspondingly, we find that the Kvβ channel subunit <jats:italic>Hyperkinetic</jats:italic> with a well conserved redox sensor domain links light-evoked redox changes in CRY to rapid changes in membrane electrical potential. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 112 (7), 2245-2250, 2015-02-02
Proceedings of the National Academy of Sciences