Solubility measurements by in situ observation of the apex region formed by the (110), (11̄0) and (101) faces of tetragonal lysozyme crystals

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Abstract Equilibrium temperatures, T e , of tetragonal hen egg-white lysozyme crystals were determined by in situ observation of apex regions formed by the (110), (110) and (101) faces of the crystals. We could precisely measure T e within 60 min, and with an error margin ±0.7 °C or less. This is largely because growth and dissolution can be detected more rapidly at the apex than at the steps at the center of the crystal faces.

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