A Polysaccharide Deacetylase Gene ( <i>pdaA</i> ) Is Required for Germination and for Production of Muramic δ-Lactam Residues in the Spore Cortex of <i>Bacillus subtilis</i>

DOI Web Site PubMed 被引用文献3件 オープンアクセス
  • Tatsuya Fukushima
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda-shi, Nagano 386, Japan
  • Hiroki Yamamoto
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda-shi, Nagano 386, Japan
  • Abdelmadjid Atrih
    Department of Molecular Biology and Biotechnology, University of Sheffield, Fifth Court, Western Bank, Sheffield S10 2TN, United Kingdom
  • Simon J. Foster
    Department of Molecular Biology and Biotechnology, University of Sheffield, Fifth Court, Western Bank, Sheffield S10 2TN, United Kingdom
  • Junichi Sekiguchi
    Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda-shi, Nagano 386, Japan

この論文をさがす

説明

<jats:title>ABSTRACT</jats:title> <jats:p> The predicted amino acid sequence of <jats:italic>Bacillus subtilis yfjS</jats:italic> (renamed <jats:italic>pdaA</jats:italic> ) exhibits high similarity to those of several polysaccharide deacetylases. β-Galactosidase fusion experiments and results of Northern hybridization with sporulation sigma mutants indicated that the <jats:italic>pdaA</jats:italic> gene is transcribed by Eσ <jats:sup>G</jats:sup> RNA polymerase. <jats:italic>pdaA</jats:italic> -deficient spores were bright by phase-contrast microscopy, and the spores were induced to germination on the addition of <jats:sc>l</jats:sc> -alanine. Germination-associated spore darkening, a slow and partial decrease in absorbance, and slightly lower dipicolinic acid release compared with that by the wild-type strain were observed. In particular, the release of hexosamine-containing materials was lacking in the <jats:italic>pdaA</jats:italic> mutant. Muropeptide analysis indicated that the <jats:italic>pdaA</jats:italic> -deficient spores completely lacked muramic δ-lactam. A <jats:italic>pdaA-gfp</jats:italic> fusion protein constructed in strain 168 and <jats:italic>pdaA</jats:italic> -deficient strains indicated that the protein is localized in <jats:italic>B. subtilis</jats:italic> spores. The biosynthetic pathway of muramic δ-lactam is discussed. </jats:p>

収録刊行物

被引用文献 (3)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ