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- Ido Yofe
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Kareem Soliman
- University Medical Center 2 Department of Child and Adolescent Health , , Göttingen 37075 , Germany
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- Silvia G. Chuartzman
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Bruce Morgan
- University of Kaiserslautern 3 Department of Cellular Biochemistry , , Kaiserslautern 67653 , Germany
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- Uri Weill
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Eden Yifrach
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Tobias P. Dick
- ZMBH-DKFZ Alliance, German Cancer Research Center (DKFZ) 4 Division of Redox Regulation , , Heidelberg 69121 , Germany
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- Sara J. Cooper
- HudsonAlpha Institute for Biotechnology 5 , Huntsville, AL 35806 , USA
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- Christer S. Ejsing
- VILLUM Center for Bioanalytical Sciences, University of Southern Denmark 6 Department of Biochemistry and Molecular Biology , , Odense 5230 , Denmark
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- Maya Schuldiner
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Einat Zalckvar
- Weizmann Institute of Science 1 Department of Molecular Genetics , , Rehovot 7610001 , Israel
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- Sven Thoms
- University Medical Center 2 Department of Child and Adolescent Health , , Göttingen 37075 , Germany
書誌事項
- 公開日
- 2017-02-15
- 権利情報
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- http://www.biologists.com/user-licence-1-1/
- DOI
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- 10.1242/jcs.187914
- 公開者
- The Company of Biologists
この論文をさがす
説明
<jats:title>ABSTRACT</jats:title> <jats:p>Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by PEX genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in Saccharomyces cerevisiae to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term PEX35, which affects peroxisome abundance. PEX35 encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in PEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.</jats:p>
収録刊行物
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- Journal of Cell Science
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Journal of Cell Science 130 (4), 791-804, 2017-02-15
The Company of Biologists