PIL5, a Phytochrome-Interacting bHLH Protein, Regulates Gibberellin Responsiveness by Binding Directly to the<i>GAI</i>and<i>RGA</i>Promoters in<i>Arabidopsis</i>Seeds

DOI PDF 被引用文献11件
  • Eunkyoo Oh
    Department of Biological Sciences, KAIST, Daejeon 305-701, Korea
  • Shinjiro Yamaguchi
    RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan
  • Jianhong Hu
    Department of Biology, Duke University, Durham, North Carolina 27708-1000
  • Jikumaru Yusuke
    RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan
  • Byunghyuck Jung
    Department of Chemistry, KAIST, Daejeon 305-701, Korea
  • Inyup Paik
    Department of Biological Sciences, KAIST, Daejeon 305-701, Korea
  • Hee-Seung Lee
    Department of Chemistry, KAIST, Daejeon 305-701, Korea
  • Tai-ping Sun
    Department of Biology, Duke University, Durham, North Carolina 27708-1000
  • Yuji Kamiya
    RIKEN Plant Science Center, Yokohama, Kanagawa 230-0045, Japan
  • Giltsu Choi
    Department of Biological Sciences, KAIST, Daejeon 305-701, Korea

書誌事項

公開日
2007-04-01
権利情報
  • https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
DOI
  • 10.1105/tpc.107.050153
公開者
Oxford University Press (OUP)

説明

<jats:title>Abstract</jats:title><jats:p>Previous work showed that PHYTOCHROME-INTERACTING FACTOR3-LIKE5 (PIL5), a light-labile basic helix-loop-helix protein, inhibits seed germination by repressing GIBBERELLIN 3β-HYDROXYLASE1 (GA3ox1) and GA3ox2 and activating a gibberellic acid (GA) catabolic gene (GA2ox2). However, we show persistent light-dependent and PIL5-inhibited germination behavior in the absence of both de novo GA biosynthesis and deactivation by GA2ox2, suggesting that PIL5 regulates not only GA metabolism but also GA responsiveness. PIL5 increases the expression of two GA repressor (DELLA) genes, GA-INSENSITIVE (GAI) and REPRESSOR OF GA1-3 (RGA/RGA1), in darkness. The hypersensitivity of gai-t6 rga-28 to red light and the suppression of germination defects of a rga-28 PIL5 overexpression line show the significant role of this transcriptional regulation in seed germination. PIL5 also increases abscisic acid (ABA) levels by activating ABA biosynthetic genes and repressing an ABA catabolic gene. PIL5 binds directly to GAI and RGA promoters but not to GA and ABA metabolic gene promoters. Together, our results show that light signals perceived by phytochromes cause a reduction in the PIL5 protein level, which in turn regulates the transcription of two DELLA genes directly and that of GA and ABA metabolic genes indirectly.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 19 (4), 1192-1208, 2007-04-01

    Oxford University Press (OUP)

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