5-HT<sub>2A</sub>receptors: location and functional analysis in intestines of wild-type and 5-HT<sub>2A</sub>knockout mice

DOI Web Site 被引用文献5件
  • Elena Fiorica-Howells
    Departments of Anatomy and Cell Biology and
  • Rene Hen
    Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
  • Jay Gingrich
    Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
  • Zhishan Li
    Departments of Anatomy and Cell Biology and
  • Michael D. Gershon
    Departments of Anatomy and Cell Biology and

書誌事項

公開日
2002-05-01
DOI
  • 10.1152/ajpgi.00435.2001
公開者
American Physiological Society

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説明

<jats:p>The distribution and function of the 5-hydroxytryptamine (5-HT<jats:sub>2A</jats:sub>) receptor were investigated in the intestines of wild-type (5-HT<jats:sub>2A</jats:sub>+/+) and knockout (5-HT<jats:sub>2A</jats:sub>−/−) mice. In 5-HT<jats:sub>2A</jats:sub>+/+ mice, rats, and guinea pigs, 5-HT<jats:sub>2A</jats:sub>receptor immunoreactivity was found on circular and longitudinal smooth muscle cells, neurons, enterocytes, and Paneth cells. Muscular 5-HT<jats:sub>2A</jats:sub>receptors were concentrated in caveolae; neuronal 5-HT<jats:sub>2A</jats:sub>receptors were found intracellularly and on the plasma membranes of nerve cell bodies and axons. Neuronal 5-HT<jats:sub>2A</jats:sub>immunoreactivity was detected as early as E14 in ganglia, intravillus nerves, and the deep muscle plexus. The 5-HT<jats:sub>2A</jats:sub>−/− colon did not express 5-HT<jats:sub>2A</jats:sub>receptors and did not contract in response to exogenous 5-HT. 5-HT<jats:sub>2A</jats:sub>−/− enterocytes were smaller, Paneth cells fewer, and muscle layers thinner (and showed degeneration) compared with those of 5-HT<jats:sub>2A</jats:sub>+/+ littermates. The 5-HT<jats:sub>2A</jats:sub>receptor may thus be required for the maintenance and/or development of enteric neuroeffectors and other enteric functions, although gastrointestinal and colonic transit times in 5-HT<jats:sub>2A</jats:sub>−/− and +/+ mice did not differ significantly.</jats:p>

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