Strain-specific parallel evolution drives short-term diversification during <i>Pseudomonas aeruginosa</i> biofilm formation
-
- Kerensa E. McElroy
- School of Biotechnology and Biomolecular Sciences and
-
- Janice G. K. Hui
- School of Biotechnology and Biomolecular Sciences and
-
- Jerry K. K. Woo
- School of Biotechnology and Biomolecular Sciences and
-
- Alison W. S. Luk
- School of Biotechnology and Biomolecular Sciences and
-
- Jeremy S. Webb
- Centre for Biological Sciences and Institute of Life Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom;
-
- Staffan Kjelleberg
- School of Biotechnology and Biomolecular Sciences and
-
- Scott A. Rice
- School of Biotechnology and Biomolecular Sciences and
-
- Torsten Thomas
- School of Biotechnology and Biomolecular Sciences and
説明
<jats:title>Significance</jats:title> <jats:p> Within-population genetic diversity is an essential evolutionary prerequisite for processes ranging from antibiotic resistance to niche adaptation, but its generation is poorly understood, with most studies focusing on fixed substitutions at the end point of long-term evolution. Using deep sequencing, we analyzed short-term, within-population genetic diversification occurring during biofilm formation of the model bacterium <jats:italic>Pseudomonas aeruginosa</jats:italic> . We discovered extensive parallel evolution between biological replicates at the level of pathways, genes, and even individual nucleotides. Short-term diversification featured positive selection of relatively few nonsynonymous mutations, with the majority of the genome being conserved by negative selection. This result is broadly consistent with observations of long-term evolution and suggests diversifying selection may underlie genetic diversification of <jats:italic>Pseudomonas aeruginosa</jats:italic> biofilms. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 111 (14), E1419-, 2014-03-28
Proceedings of the National Academy of Sciences