<i>De Novo</i>Formation of Adipose Tissue by Controlled Release of Basic Fibroblast Growth Factor
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- Yasuhiko Tabata
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
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- Manabu Miyao
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
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- Takashi Inamoto
- College of Medical Technology, Kyoto University, Kyoto 606-8507, Japan.
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- Toshihiro Ishii
- Department of Applied Chemistry, Osaka Institute of Technology, Osaka 535-8585, Japan.
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- Yoshiaki Hirano
- Department of Applied Chemistry, Osaka Institute of Technology, Osaka 535-8585, Japan.
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- Yoshio Yamaoki
- Department of Gastroenterological Surgery, Graduate School of Medicine, Kyoto 606-8501, Japan.
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- Yoshito Ikada
- Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
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説明
De novo adipogenesis at the implanted site of a basement membrane extract (Matrigel) was induced through controlled release of basic fibroblast growth factor (bFGF). bFGF was incorporated into biodegradable gelatin microspheres for its controlled release. When the mixture of Matrigel and bFGF-incorporated gelatin microspheres was implanted subcutaneously into the back of mice, a clearly visible fat pad was formed at the implanted site 6 weeks later. Histologic examination revealed that the de novo formation of adipose tissue accompanied with angiogenesis was observed in the implanted Matrigel at bFGF doses of 0.01, 0.1, and 1 microg/site, the lower and higher doses being less effective. The de novo formation induced by the bFGF-incorporated microspheres was significantly higher than that induced by free bFGF of the same dose. The mRNA of a lipogenesis marker protein, glycerophosphate dehydrogenase, was detected in the formed adipose tissues, biochemically indicating de novo adipogenesis. Free bFGF, the bFGF-incorporated gelatin microspheres, or Marigel alone and bFGF-free gelatin microspheres with or without Matrigel did not induce formation of adipose tissue. This de novo adipogenesis by mixture of Matrigel and the bFGF-incorporated gelatin microspheres will provide a new idea for tissue engineering of adipose tissue.
収録刊行物
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- Tissue Engineering
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Tissue Engineering 6 (3), 279-289, 2000-06
Mary Ann Liebert Inc
