A Phosphorylated Cytoplasmic Autoantigen, GW182, Associates with a Unique Population of Human mRNAs within Novel Cytoplasmic Speckles
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- Theophany Eystathioy
- Departments of Medicine and Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta AB T2N 4N1, Canada;
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- Edward K. L. Chan
- W.M. Keck Autoimmune Disease Center, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037;
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- Scott A. Tenenbaum
- Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710
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- Jack D. Keene
- Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710
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- Kevin Griffith
- Departments of Medicine and Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta AB T2N 4N1, Canada;
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- Marvin J. Fritzler
- Departments of Medicine and Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta AB T2N 4N1, Canada;
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- Joseph Gall
- editor
Description
<jats:p>A novel human cellular structure has been identified that contains a unique autoimmune antigen and multiple messenger RNAs. This complex was discovered using an autoimmune serum from a patient with motor and sensory neuropathy and contains a protein of 182 kDa. The gene and cDNA encoding the protein indicated an open reading frame with glycine-tryptophan (GW) repeats and a single RNA recognition motif. Both the patient's serum and a rabbit serum raised against the recombinant GW protein costained discrete cytoplasmic speckles designated as GW bodies (GWBs) that do not overlap with the Golgi complex, endosomes, lysosomes, or peroxisomes. The mRNAs associated with GW182 represent a clustered set of transcripts that are presumed to reside within the GW complexes. We propose that the GW ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene transcripts involved in cell growth and homeostasis.</jats:p>
Journal
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- Molecular Biology of the Cell
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Molecular Biology of the Cell 13 (4), 1338-1351, 2002-04
American Society for Cell Biology (ASCB)
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Details 詳細情報について
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- CRID
- 1360574096102703744
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- NII Article ID
- 30018378146
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- ISSN
- 19394586
- 10591524
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- Data Source
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- Crossref
- CiNii Articles