Live imaging of neural structure and function by fibred fluorescence microscopy
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- Pierre Vincent
- Université Pierre et Marie Curie‐Paris, ERT 1059, Boîte Courrier number 16 9 Quai St Bernard 75005 Paris France
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- Uwe Maskos
- Récepteurs et Cognition, CNRS URA 2182, Institut Pasteur 25 Rue du Dr Roux 75724 Paris Cedex 15 France
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- Igor Charvet
- Department of Cell Physiology and Metabolism, University of Geneva 1 Rue Michel Servet 1211 Geneva 4 Switzerland
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- Laurence Bourgeais
- Université Pierre et Marie Curie‐Paris, ERT 1059, Boîte Courrier number 16 9 Quai St Bernard 75005 Paris France
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- Luc Stoppini
- BioCell Interface, Rue de la Serre 91, CP 131 2301 La Chaux‐de‐Fonds Switzerland
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- Nathalie Leresche
- Université Pierre et Marie Curie‐Paris, ERT 1059, Boîte Courrier number 16 9 Quai St Bernard 75005 Paris France
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- Jean‐Pierre Changeux
- Récepteurs et Cognition, CNRS URA 2182, Institut Pasteur 25 Rue du Dr Roux 75724 Paris Cedex 15 France
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- Régis Lambert
- Université Pierre et Marie Curie‐Paris, ERT 1059, Boîte Courrier number 16 9 Quai St Bernard 75005 Paris France
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- Paolo Meda
- Department of Cell Physiology and Metabolism, University of Geneva 1 Rue Michel Servet 1211 Geneva 4 Switzerland
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- Danièle Paupardin‐Tritsch
- Université Pierre et Marie Curie‐Paris, ERT 1059, Boîte Courrier number 16 9 Quai St Bernard 75005 Paris France
抄録
<jats:p>Only a few methods permit researchers to study selected regions of the central and peripheral nervous systems with a spatial and time resolution sufficient to image the function of neural structures. Usually, these methods cannot analyse deep‐brain regions and a high‐resolution method, which could repeatedly probe dynamic processes in any region of the central and peripheral nervous systems, is much needed. Here, we show that fibred fluorescence microscopy—which uses a small‐diameter fibre‐optic probe to provide real‐time images—has the spatial resolution to image various neural structures in the living animal, the consistency needed for a sequential, quantitative evaluation of axonal degeneration/regeneration of a peripheral nerve, and the sensitivity to detect calcium transients on a sub‐second timescale. These unique features should prove useful in many physiological studies requiring the <jats:italic>in situ</jats:italic> functional imaging of tissues in a living animal.</jats:p>
収録刊行物
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- EMBO reports
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EMBO reports 7 (11), 1154-1161, 2006-09-29
Springer Science and Business Media LLC