A lysin motif effector subverts chitin‐triggered immunity to facilitate arbuscular mycorrhizal symbiosis
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- Tian Zeng
- Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Luis Rodriguez‐Moreno
- Department of Plant Sciences Laboratory of Phytopathology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Artem Mansurkhodzaev
- Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Peng Wang
- Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Willy van den Berg
- Laboratory of Biochemistry Wageningen University & Research 6708 WE Wageningen the Netherlands
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- Virginie Gasciolli
- LIPM Université de Toulouse INRA CNRS Castanet‐Tolosan France
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- Sylvain Cottaz
- CNRS CERMAV University Grenoble Alpes UPR 5301 38041 Grenoble France
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- Sébastien Fort
- CNRS CERMAV University Grenoble Alpes UPR 5301 38041 Grenoble France
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- Bart P. H. J. Thomma
- Department of Plant Sciences Laboratory of Phytopathology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Jean‐Jacques Bono
- LIPM Université de Toulouse INRA CNRS Castanet‐Tolosan France
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- Ton Bisseling
- Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands
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- Erik Limpens
- Laboratory of Molecular Biology Wageningen University & Research 6708 PB Wageningen the Netherlands
Abstract
<jats:title>Summary</jats:title><jats:p> <jats:list list-type="bullet"> <jats:list-item><jats:p>Arbuscular mycorrhizal (AM) fungi greatly improve mineral uptake by host plants in nutrient‐depleted soil and can intracellularly colonize root cortex cells in the vast majority of higher plants. However, AM fungi possess common fungal cell wall components such as chitin that can be recognized by plant chitin receptors to trigger immune responses, raising the question as to how AM fungi effectively evade chitin‐triggered immune responses during symbiosis.</jats:p></jats:list-item> <jats:list-item><jats:p>In this study, we characterize a secreted lysin motif (LysM) effector identified from the model AM fungal species <jats:italic>Rhizophagus irregularis</jats:italic>, called RiSLM.</jats:p></jats:list-item> <jats:list-item><jats:p>RiSLM is one of the highest expressed effector proteins in intraradical mycelium during the symbiosis. <jats:italic>In vitro</jats:italic> binding assays show that RiSLM binds chitin‐oligosaccharides and can protect fungal cell walls from chitinases. Moreover, RiSLM efficiently interferes with chitin‐triggered immune responses, such as defence gene induction and reactive oxygen species production in <jats:italic>Medicago truncatula.</jats:italic> Although RiSLM also binds to symbiotic (lipo)chitooligosaccharides it does not interfere significantly with symbiotic signalling in <jats:italic>Medicago</jats:italic>. Host‐induced gene silencing of <jats:italic>RiSLM</jats:italic> greatly reduces fungal colonization levels.</jats:p></jats:list-item> <jats:list-item><jats:p>Taken together, our results reveal a key role for AM fungal LysM effectors to subvert chitin‐triggered immunity in symbiosis, pointing to a common role for LysM effectors in both symbiotic and pathogenic fungi.</jats:p></jats:list-item> </jats:list> </jats:p>
Journal
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- New Phytologist
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New Phytologist 225 (1), 448-460, 2019-11-23
Wiley
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Details 詳細情報について
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- CRID
- 1360574096444246528
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- ISSN
- 14698137
- 0028646X
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- Data Source
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- Crossref