Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
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- Mizuki Ogawa
- Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan
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- Miyako Udono
- Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan
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- Kiichiro Teruya
- Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan
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- Norihisa Uehara
- Department of Molecular Cell Biology and Oral Anatomy, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan
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- Yoshinori Katakura
- Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan
説明
<jats:p>Enhanced telomerase reverse transcriptase (TERT) levels in dermal keratinocytes can serve as a novel target for hair growth promotion. Previously, we identified fisetin using a system for screening food components that can activate the TERT promoter in HaCaT cells (keratinocytes). In the present study, we aimed to clarify the molecular basis of fisetin-induced hair growth promotion in mice. To this end, the dorsal skin of mice was treated with fisetin, and hair growth was evaluated 12 days after treatment. Histochemical analyses of fisetin-treated skin samples and HaCaT cells were performed to observe the effects of fisetin. The results showed that fisetin activated HaCaT cells by regulating the expression of various genes related to epidermogenesis, cell proliferation, hair follicle regulation, and hair cycle regulation. In addition, fisetin induced the secretion of exosomes from HaCaT cells, which activated β-catenin and mitochondria in hair follicle stem cells (HFSCs) and induced their proliferation. Moreover, these results revealed the existence of exosomes as the molecular basis of keratinocyte-HFSC interaction and showed that fisetin, along with its effects on keratinocytes, caused exosome secretion, thereby activating HFSCs. This is the first study to show that keratinocyte-derived exosomes can activate HFSCs and consequently induce hair growth.</jats:p>
収録刊行物
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- Nutrients
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Nutrients 13 (6), 2087-, 2021-06-18
MDPI AG
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キーワード
- Keratinocytes
- Flavonols
- exosomes
- Exosomes
- Mice
- Sirtuin 1
- Skin Physiological Phenomena
- Animals
- HaCaT Cells
- Humans
- hair cycle regulation
- Telomerase
- Cell Proliferation
- Skin
- Stem Cells
- telogen–anagen transition
- telomerase reverse transcriptase
- keratinocyte–hair follicle stem cell interaction
- Mice, Inbred C57BL
- Female
- Hair Follicle
- Hair
詳細情報 詳細情報について
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- CRID
- 1360576118799372288
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- ISSN
- 20726643
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- PubMed
- 34207142
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- 資料種別
- journal article
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- データソース種別
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- Crossref
- KAKEN
- OpenAIRE
