Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors in Bombyx mori

DOI Web Site 被引用文献2件 参考文献38件 オープンアクセス
  • Yudai Masuoka
    Insect Design Technology Module, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba 305-8634, Ibaraki, Japan
  • Wei Cao
    Research Center for Agricultural Information Technology (RCAIT), National Agriculture and Food Research Organization (NARO), 1-31-1 Kannondai, Tsukuba 305-0856, Ibaraki, Japan
  • Akiya Jouraku
    Insect Design Technology Module, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba 305-8634, Ibaraki, Japan
  • Hiroki Sakai
    Silkworm Research Module, Division of Silk-Producing Insect Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba 305-8634, Ibaraki, Japan
  • Hideki Sezutsu
    Silkworm Research Module, Division of Silk-Producing Insect Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba 305-8634, Ibaraki, Japan
  • Kakeru Yokoi
    Insect Design Technology Module, Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), 1-2 Owashi, Tsukuba 305-8634, Ibaraki, Japan

説明

<jats:p>Bombyx mori is an important economic insect and an animal model in pharmacomedical research. Although its physiology has been studied for many years, the mechanism via which silk protein genes are regulated remains unclear. In this study, we performed two-step expression screening, namely co-expression network and time-course expression analyses to screen silk protein regulation factors. A co-expression network analysis using RNA-seq data that were obtained from various tissues, including the silk glands of B. mori, was performed to identify novel silk protein regulatory factors. Overall, 91 transcription factors, including some known ones, were found to be co-expressed with silk protein genes. Furthermore, time-course expression analysis during the fifth instar larvae stage revealed that the expression pattern of 13 novel transcription factors was highly relevant to that of silk protein genes and their known regulatory factor genes. In particular, the expression peak of several transcription factors (TFs) was detected before the expression of silk protein genes peak. These results indicated that a larger number of genes than expected may be involved in silk protein regulation in B. mori. Functional analyses of function-unknown transcription factors should enhance our understanding of this system.</jats:p>

収録刊行物

  • Insects

    Insects 13 (2), 131-, 2022-01-26

    MDPI AG

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