Testosterone therapy induces molecular programming augmenting physiological adaptations to resistance exercise in older men

  • Nima Gharahdaghi
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Supreeth Rudrappa
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Matthew S. Brook
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Iskandar Idris
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Hannah Crossland
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Claire Hamrock
    Institute of Food and Health University College Dublin, Belfield Dublin Ireland
  • Muhammad Hariz Abdul Aziz
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Fawzi Kadi
    Division of Sports Sciences, School of Health and Medical Sciences Örebro University Örebro Sweden
  • Janelle Tarum
    Division of Sports Sciences, School of Health and Medical Sciences Örebro University Örebro Sweden
  • Paul L. Greenhaff
    MRC‐ARUK Centre for Musculoskeletal Ageing Research, School of Life Sciences University of Nottingham, Nottingham Nottingham UK
  • Dumitru Constantin‐Teodosiu
    MRC‐ARUK Centre for Musculoskeletal Ageing Research, School of Life Sciences University of Nottingham, Nottingham Nottingham UK
  • Jessica Cegielski
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Bethan E. Phillips
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Daniel J. Wilkinson
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Nathaniel J. Szewczyk
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Kenneth Smith
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK
  • Philip J. Atherton
    MRC‐ARUK Centre for Musculoskeletal Ageing Research and Nottingham NIHR BRC, School of Medicine University of Nottingham, Derby UK

抄録

<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>The andropause is associated with declines in serum testosterone (T), loss of muscle mass (sarcopenia), and frailty. Two major interventions purported to offset sarcopenia are anabolic steroid therapies and resistance exercise training (RET). Nonetheless, the efficacy and physiological and molecular impacts of T therapy adjuvant to short‐term RET remain poorly defined.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Eighteen non‐hypogonadal healthy older men, 65–75 years, were assigned in a random double‐blinded fashion to receive, biweekly, either placebo (P, saline, <jats:italic>n</jats:italic> = 9) or T (Sustanon 250 mg, <jats:italic>n</jats:italic> = 9) injections over 6 week whole‐body RET (three sets of 8–10 repetitions at 80% one‐repetition maximum). Subjects underwent dual‐energy X‐ray absorptiometry, ultrasound of vastus lateralis (VL) muscle architecture, and knee extensor isometric muscle force tests; VL muscle biopsies were taken to quantify myogenic/anabolic gene expression, anabolic signalling, muscle protein synthesis (D<jats:sub>2</jats:sub>O), and breakdown (extrapolated).</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Testosterone adjuvant to RET augmented total fat‐free mass (<jats:italic>P=</jats:italic>0.007), legs fat‐free mass (<jats:italic>P=</jats:italic>0.02), and appendicular fat‐free mass (<jats:italic>P=</jats:italic>0.001) gains while decreasing total fat mass (<jats:italic>P=</jats:italic>0.02). Augmentations in VL muscle thickness, fascicle length, and quadriceps cross‐section area with RET occured to a greater extent in T (<jats:italic>P</jats:italic> < 0.05). Sum strength (<jats:italic>P=</jats:italic>0.0009) and maximal voluntary contract (e.g. knee extension at 70°) (<jats:italic>P=</jats:italic>0.002) increased significantly more in the T group. Mechanistically, both muscle protein synthesis rates (T: 2.13 ± 0.21%·day<jats:sup>−1</jats:sup> vs. P: 1.34 ± 0.13%·day<jats:sup>−1</jats:sup>, <jats:italic>P=</jats:italic>0.0009) and absolute breakdown rates (T: 140.2 ± 15.8 g·day<jats:sup>−1</jats:sup> vs. P: 90.2 ± 11.7 g·day<jats:sup>−1</jats:sup>, <jats:italic>P=</jats:italic>0.02) were elevated with T therapy, which led to higher net turnover and protein accretion in the T group (T: 8.3 ± 1.4 g·day<jats:sup>−1</jats:sup> vs. P: 1.9 ± 1.2 g·day<jats:sup>−1</jats:sup>, <jats:italic>P=</jats:italic>0.004). Increases in ribosomal biogenesis (RNA:DNA ratio); mRNA expression relating to T metabolism (androgen receptor: 1.4‐fold; <jats:italic>Srd5a1</jats:italic>: 1.6‐fold; <jats:italic>AKR1C3</jats:italic>: 2.1‐fold; and <jats:italic>HSD17β3</jats:italic>: two‐fold); insulin‐like growth factor (IGF)‐1 signalling [<jats:italic>IGF‐1Ea</jats:italic> (3.5‐fold) and <jats:italic>IGF‐1Ec</jats:italic> (three‐fold)] and myogenic regulatory factors; and the activity of anabolic signalling (e.g. mTOR, AKT, and RPS6; <jats:italic>P</jats:italic> < 0.05) were all up‐regulated with T therapy. Only T up‐regulated mitochondrial citrate synthase activity (<jats:italic>P=</jats:italic>0.03) and transcription factor A (1.41 ± 0.2‐fold, <jats:italic>P=</jats:italic>0.0002), in addition to peroxisome proliferator‐activated receptor‐γ co‐activator 1‐α mRNA (1.19 ± 0.21‐fold, <jats:italic>P=</jats:italic>0.037).</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>Administration of T adjuvant to RET enhanced skeletal muscle mass and performance, while up‐regulating myogenic gene programming, myocellular translational efficiency and capacity, collectively resulting in higher protein turnover, and net protein accretion. T coupled with RET is an effective short‐term intervention to improve muscle mass/function in older non‐hypogonadal men.</jats:p></jats:sec>

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