Antiplasmodial and Antipyretic Activity and Safety Evaluation of the Methanolic Leaf Extract of <i>Murraya exotica (L.)</i>

<jats:p><jats:italic>Background</jats:italic>. The increasing mortality and morbidity of malaria in Africa coupled with the recent reports of antimalarial drug resistance reinforces the need for novel antimalarial agents from natural plant products with folkloric use for the disease. <jats:italic>Murraya exotica (L.)</jats:italic> (Rutaceae) is widely used as an ornamental plant used indigenously to treat fever, cough, and infectious wounds and eliminate pain from injury and trauma. This study was conducted to evaluate extracts of the leaves of <jats:italic>Murraya exotica (L.)</jats:italic> (Rutaceae) for its safety and antipyretic and antimalarial activity in rodent models. <jats:italic>Method</jats:italic>. In this study, the Peters 4-day suppressive and curative test in <jats:italic>Plasmodium berghei</jats:italic>-infected mice was used to demonstrate the antiplasmodial activity of the methanolic leaf extract of <jats:italic>Murraya exotica (L.)</jats:italic> (MEE). The study also evaluated the subacute toxicity study and the antipyretic activity of MEE on baker’s yeast-induced hyperthermia in rodent models. <jats:italic>Results</jats:italic>. <jats:italic>Murraya exotica (L.)</jats:italic> extract demonstrated curative antimalarial activity, with a percentage suppression of 45.84, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mn>64.32</mml:mn><mml:mo>±</mml:mo><mml:mn>0.33</mml:mn></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mn>56.74</mml:mn><mml:mo>±</mml:mo><mml:mn>2.16</mml:mn></mml:math>, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mn>64.61</mml:mn><mml:mo>±</mml:mo><mml:mn>0.67</mml:mn></mml:math> at doses of 50, 100, 300, and 600 mg/kg, respectively. In the Peters 4-day suppressive test, MEE at dose 600 mg/kg had the highest chemosuppression (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mn>76.02</mml:mn><mml:mo>±</mml:mo><mml:mn>1.38</mml:mn><mml:mi>%</mml:mi></mml:math>) compared with artesunate (2 mg/kg, <jats:italic>p.o.</jats:italic>) (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mn>82.56</mml:mn><mml:mo>±</mml:mo><mml:mn>0.97</mml:mn><mml:mi>%</mml:mi></mml:math>). Subacute oral toxicity studies in Sprague-Dawley rats documented no deaths, with no significant changes in clinical signs, organ weights, and hematological and biochemical parameters. The LD<jats:sub>50</jats:sub> of MEE was estimated to be above 1000 mg/kg in Sprague-Dawley rats. All doses of MEE and paracetamol reduced pyrexia in 1 h and 2 h after their administration. The percentage reduction of rectal temperature (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:msub><mml:mrow><mml:mi>T</mml:mi></mml:mrow><mml:mrow><mml:mi>R</mml:mi></mml:mrow></mml:msub></mml:math>) for the positive control (paracetamol, 150 mg/kg, <jats:italic>p.o.</jats:italic>) was 44.36% while the <jats:italic>Murraya exotica</jats:italic> extract at doses 50 mg/kg, 100 mg/kg, 300 mg/kg, and 600 mg/kg recorded 67.74%, 40.78%, 66.42%, and 59.42%, respectively. <jats:italic>Murraya exotica</jats:italic> at dose 100 mg/kg exhibited significant reduction (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:math>) in baker’s yeast-induced pyrexia. <jats:italic>Conclusions</jats:italic>. The findings in this study show the antipyretic, curative, and suppressive antiplasmodial activity as well as the safety of the methanolic leaf extract of <jats:italic>Murraya exotica (L.)</jats:italic> supporting its traditional use for malaria and fever.</jats:p>