Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids

DOI Web Site 2 Citations
  • Saravanan Devendran
    Microbiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, Illinois, USA
  • Rachana Shrestha
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • João M. P. Alves
    Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
  • Patricia G. Wolf
    Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Lindsey Ly
    Microbiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, Illinois, USA
  • Alvaro G. Hernandez
    Keck Center for Biotechnology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Celia Méndez-García
    Independent Researcher, Boston, Massachusetts, USA
  • Ashley Inboden
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • J'nai Wiley
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • Oindrila Paul
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • Avery Allen
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • Emily Springer
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • Chris L. Wright
    Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Christopher J. Fields
    Division of Nutritional Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
  • Steven L. Daniel
    Department of Biological Sciences, Eastern Illinois University, Charleston, Illinois, USA
  • Jason M. Ridlon
    Microbiome Metabolic Engineering Theme, Carl R. Woese Institute for Genomic Biology, Urbana, Illinois, USA
  • Volker Müller
    editor

Description

<jats:p> <jats:named-content content-type="genus-species">C. scindens</jats:named-content> is one of a few identified gut bacterial species capable of converting host cholic acid into disease-associated secondary bile acids such as deoxycholic acid. The current work represents an important advance in understanding the nutritional requirements and response to bile acids of the medically important human gut bacterium, <jats:named-content content-type="genus-species">C. scindens</jats:named-content> ATCC 35704. A defined medium has been developed which will further the understanding of bile acid metabolism in the context of growth substrates, cofactors, and other metabolites in the vertebrate gut. Analysis of the complete genome supports the nutritional requirements reported here. Genome-wide transcriptomic analysis of gene expression in the presence of cholic acid and deoxycholic acid provides a unique insight into the complex response of <jats:named-content content-type="genus-species">C. scindens</jats:named-content> ATCC 35704 to primary and secondary bile acids. Also revealed are genes with the potential to function in bile acid transport and metabolism. </jats:p>

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