In Vitro and In Vivo Models for Studying SARS-CoV-2, the Etiological Agent Responsible for COVID-19 Pandemic
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- Rafael B. Rosa
- Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), Recife 50740-465, Brazil
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- Willyenne M. Dantas
- Department of Chemistry, Federal Rural University of Pernambuco (UFRPE), Recife 52171-900, Brazil
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- Jessica C. F. do Nascimento
- Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), Recife 50740-465, Brazil
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- Murilo V. da Silva
- Rodents Animal Facilities Complex, Federal University of Uberlandia, Uberlandia 38400-902, Brazil
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- Ronaldo N. de Oliveira
- Department of Chemistry, Federal Rural University of Pernambuco (UFRPE), Recife 52171-900, Brazil
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- Lindomar J. Pena
- Department of Virology, Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), Recife 50740-465, Brazil
説明
<jats:p>The emergence and rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prompted the scientific community to rapidly develop in vitro and in vivo models that could be applied in COVID-19 research. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures derived from lung, alveoli, bronchi, and other organs. Although cell-based systems are economic and allow strict control of experimental variables, they do not always resemble physiological conditions. Thus, several in vivo models are being developed, including different strains of mice, hamsters, ferrets, dogs, cats, and non-human primates. In this review, we summarize the main models of SARS-CoV-2 infection developed so far and discuss their advantages, drawbacks and main uses.</jats:p>
収録刊行物
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- Viruses
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Viruses 13 (3), 379-, 2021-02-27
MDPI AG
