Dissecting specific and global transcriptional regulation of bacterial gene expression
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- Luca Gerosa
- Institute of Molecular Systems Biology, ETH Zurich Zurich Switzerland
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- Karl Kochanowski
- Institute of Molecular Systems Biology, ETH Zurich Zurich Switzerland
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- Matthias Heinemann
- Institute of Molecular Systems Biology, ETH Zurich Zurich Switzerland
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- Uwe Sauer
- Institute of Molecular Systems Biology, ETH Zurich Zurich Switzerland
Description
<jats:p>Gene expression is regulated by specific transcriptional circuits but also by the global expression machinery as a function of growth. Simultaneous specific and global regulation thus constitutes an additional—but often neglected—layer of complexity in gene expression. Here, we develop an experimental‐computational approach to dissect specific and global regulation in the bacterium <jats:italic>Escherichia coli</jats:italic>. By using fluorescent promoter reporters, we show that global regulation is growth rate dependent not only during steady state but also during dynamic changes in growth rate and can be quantified through two promoter‐specific parameters. By applying our approach to arginine biosynthesis, we obtain a quantitative understanding of both specific and global regulation that allows accurate prediction of the temporal response to simultaneous perturbations in arginine availability and growth rate. We thereby uncover two principles of joint regulation: (i) specific regulation by repression dominates the transcriptional response during metabolic steady states, largely repressing the biosynthesis genes even when biosynthesis is required and (ii) global regulation sets the maximum promoter activity that is exploited during the transition between steady states.</jats:p>
Journal
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- Molecular Systems Biology
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Molecular Systems Biology 9 (1), 658-, 2013-01
Springer Science and Business Media LLC
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Details 詳細情報について
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- CRID
- 1360576123059181824
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- ISSN
- 17444292
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- Data Source
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- Crossref