Functional primordial germ cell–like cells from pluripotent stem cells in rats

DOI Web Site 被引用文献4件 参考文献34件
  • Mami Oikawa
    Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Hisato Kobayashi
    Department of Embryology, Nara Medical University, Kashihara, Nara 634-0813, Japan.
  • Makoto Sanbo
    Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
  • Naoaki Mizuno
    Division of Stem Cell Therapy, Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Kenyu Iwatsuki
    Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Tomoya Takashima
    Department of Embryology, Nara Medical University, Kashihara, Nara 634-0813, Japan.
  • Keiko Yamauchi
    Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
  • Fumika Yoshida
    Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
  • Takuya Yamamoto
    Center for iPS Cell Research and Application, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan.
  • Takashi Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
  • Hiromitsu Nakauchi
    Division of Stem Cell Therapy, Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
  • Kazuki Kurimoto
    Department of Embryology, Nara Medical University, Kashihara, Nara 634-0813, Japan.
  • Masumi Hirabayashi
    Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
  • Toshihiro Kobayashi
    Division of Mammalian Embryology, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.

説明

<jats:p>The in vitro generation of germ cells from pluripotent stem cells (PSCs) can have a substantial effect on future reproductive medicine and animal breeding. A decade ago, in vitro gametogenesis was established in the mouse. However, induction of primordial germ cell–like cells (PGCLCs) to produce gametes has not been achieved in any other species. Here, we demonstrate the induction of functional PGCLCs from rat PSCs. We show that epiblast-like cells in floating aggregates form rat PGCLCs. The gonadal somatic cells support maturation and epigenetic reprogramming of the PGCLCs. When rat PGCLCs are transplanted into the seminiferous tubules of germline-less rats, functional spermatids—that is, those capable of siring viable offspring—are generated. Insights from our rat model will elucidate conserved and divergent mechanisms essential for the broad applicability of in vitro gametogenesis.</jats:p>

収録刊行物

  • Science

    Science 376 (6589), 176-179, 2022-04-08

    American Association for the Advancement of Science (AAAS)

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