Gene dosage compensation of rRNA transcript levels in<i>Arabidopsis thaliana</i>lines with reduced ribosomal gene copy number

DOI PDF PDF 被引用文献1件
  • Francesca B Lopez
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Antoine Fort
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Luca Tadini
    Dipartimento di Bioscienze, Universit� degli Studi di Milano, 20133 Milano, Italy
  • Aline V Probst
    CNRS, GReD, Universit� Clermont Auvergne, INSERM, 63001 Clermont–Ferrand, France
  • Marcus McHale
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • James Friel
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Peter Ryder
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Fr�d�ric Pontvianne
    CNRS, Laboratoire G�nome et D�veloppement des Plantes (LGDP), Universit� de Perpignan Via Domitia, Perpignan, France
  • Paolo Pesaresi
    Dipartimento di Bioscienze, Universit� degli Studi di Milano, 20133 Milano, Italy
  • Ronan Sulpice
    Systems Biology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Peter McKeown
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Galina Brychkova
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland
  • Charles Spillane
    Genetics and Biotechnology Laboratory, Plant & AgriBiosciences Research Centre (PABC), Ryan Institute, National University of Ireland Galway, Galway H91 REW4, Ireland

抄録

<jats:title>Abstract</jats:title><jats:p>The 45S rRNA genes (rDNA) are among the largest repetitive elements in eukaryotic genomes. rDNA consists of tandem arrays of rRNA genes, many of which are transcriptionally silenced. Silent rDNA repeats may act as ‘back-up’ copies for ribosome biogenesis and have nuclear organization roles. Through Cas9-mediated genome editing in the Arabidopsis thaliana female gametophyte, we reduced 45S rDNA copy number (CN) to a plateau of ∼10%. Two independent lines had rDNA CNs reduced by up to 90% at the T7 generation, named low copy number (LCN) lines. Despite drastic reduction of rDNA copies, rRNA transcriptional rates, and steady-state levels remained the same as wild-type plants. Gene dosage compensation of rRNA transcript levels was associated with reduction of silencing histone marks at rDNA loci and altered Nucleolar Organiser Region 2 organization. Although overall genome integrity of LCN lines appears unaffected, a chromosome segmental duplication occurred in one of the lines. Transcriptome analysis of LCN seedlings identified several shared dysregulated genes and pathways in both independent lines. Cas9 genome editing of rRNA repeats to generate LCN lines provides a powerful technique to elucidate rDNA dosage compensation mechanisms and impacts of low rDNA CN on genome stability, development, and cellular processes.</jats:p>

収録刊行物

  • The Plant Cell

    The Plant Cell 33 (4), 1135-1150, 2021-02-02

    Oxford University Press (OUP)

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