One-step protocol for assays of total and direct bilirubin with stable combined reagents.
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- P Fossati
- Research & Development Laboratory, Miles Italiana SpA, Cavenago Brianza, Milan, Italy
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- M Ponti
- Research & Development Laboratory, Miles Italiana SpA, Cavenago Brianza, Milan, Italy
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- L Prencipe
- Research & Development Laboratory, Miles Italiana SpA, Cavenago Brianza, Milan, Italy
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- G Tarenghi
- Research & Development Laboratory, Miles Italiana SpA, Cavenago Brianza, Milan, Italy
書誌事項
- 公開日
- 1989-01-01
- 権利情報
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- https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model
- DOI
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- 10.1093/clinchem/35.1.173
- 公開者
- Oxford University Press (OUP)
この論文をさがす
説明
<jats:title>Abstract</jats:title> <jats:p>We describe an improved colorimetric method for assays of total and direct bilirubin in serum. Bilirubin reacts with diazotized sulfanilic acid in an acidic medium to form a blue azopigment. Total bilirubin is assayed in the presence of reaction accelerators (caffeine, urea, and citric acid), direct bilirubin in their absence. The azo compound so formed is read at the same wavelength (570 nm) in both assays. A sample blank is run in parallel. Standard curves are linear for total and direct bilirubin concentrations up to 513.0 and 256.5 mumol/L, respectively. The method is characterized by (a) use of the same protocol for both assays, i.e., a one-step procedure with short reaction time (5 min at room temperature), and (b) use of a single working solution, which, refrigerated, is stable for one month. The method is reliable, yields results that compare closely with those of the classical Jendrassik--Gróf method, is suitable for routine use, and lends itself to automation.</jats:p>
収録刊行物
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- Clinical Chemistry
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Clinical Chemistry 35 (1), 173-176, 1989-01-01
Oxford University Press (OUP)
