Two better cell lines for making hybridomas expressing specific T cell receptors.

  • J White
    Howard Hughes Medical Institute, Department of Pediatrics , Denver, CO 80206
  • M Blackman
    Howard Hughes Medical Institute, Department of Pediatrics , Denver, CO 80206
  • J Bill
    National Jewish Center for Immunology and Respiratory Medicine , Denver, CO 80206
  • J Kappler
    Department of Microbiology and Immunology, University of Colorado Health Sciences Center , Denver, CO 80262
  • P Marrack
    Department of Microbiology and Immunology, University of Colorado Health Sciences Center , Denver, CO 80262
  • D P Gold
    Medical Biology Institute , La Jolla, CA 92037
  • W Born
    National Jewish Center for Immunology and Respiratory Medicine , Denver, CO 80206

書誌事項

公開日
1989-09
権利情報
  • https://academic.oup.com/pages/standard-publication-reuse-rights
DOI
  • 10.4049/jimmunol.143.6.1822
公開者
Oxford University Press (OUP)

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説明

<jats:title>Abstract</jats:title> <jats:p>Two variants of the AKR thymoma BW5147 have been isolated which can no longer express functional TCR alpha- and beta-chains. By generating hybridomas with these variant fusion lines, TCR of any normal T lymphocyte, including TCR-gamma/delta, can be studied at a clonal level, without interference of the BW5147-derived receptor chains. In this study one of the variants has been useful in identifying the reactivity to allogeneic MHC Ag of BW5147 itself.</jats:p>

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