Exosome-like vesicles released from ob/ob mouse adipose tissue enhance cell survival of cells with radiation-induced genomic instability

DOI PDF 参考文献12件 オープンアクセス
  • Kentaro Ariyoshi
    Fukushima Medical University Integrated Center for Science and Humanities, , 1 Hikariga-oka, Fukushima, Fukushima, 960-1295 , Japan
  • Yohei Fujishima
    Institute of Radiation Emergency Medicine, Hirosaki University Department of Risk Analysis and Biodosimetry, , 66-1 Hon-cho, Hirosaki City, Aomori 036-8564 , Japan
  • Valerie Swee Ting Goh
    National University of Singapore Department of Radiobiology, Singapore Nuclear Research and Safety Initiative, , Singapore 138602 , Singapore
  • Akifumi Nakata
    Hokkaido University of Science Faculty of Pharmaceutical Sciences, , Sapporo City, Hokkaido 006-8585 , Japan
  • Kosuke Kasai
    Hirosaki University Graduate School of Health Sciences Department of Bioscience and Laboratory Medicine, , Hirosaki City, Aomori 036-8564 , Japan
  • Mitsuaki A Yoshida
    Institute of Chromosome Life Science (ICLS) ,,  Fujimino City, Saitama 356-0031   Japan
  • Tomisato Miura
    Institute of Radiation Emergency Medicine, Hirosaki University Department of Risk Analysis and Biodosimetry, , 66-1 Hon-cho, Hirosaki City, Aomori 036-8564 , Japan

抄録

<jats:title>Abstract</jats:title><jats:p>Multiple epidemiological studies have shown that obesity is a serious risk factor for cancer development. While the underlying mechanisms between obesity and cancer are still unknown, obesity disrupts the role of adipocytes in energy homeostasis, and the alteration of adipokine, insulin and sex steroid signaling. Recently, it has been identified that adipose tissue-derived exosome-like vesicles (ELVs) regulate metabolic homeostasis. In this study, we collected ELVs from adipose tissue of an obese mouse (ob/ob) strain and control mouse (C57BL/6) strain, and checked whether adipose ELVs influence radiation-induced cell death on mouse fibroblast cells (m5S). Furthermore, we analyzed the micronucleus (MN) frequency in survived cells after radiation exposure to investigate the effect of ELVs on radiation-induced genomic instability. We first observed that ELVs from control and obese mice showed enhanced colony forming ability in un-irradiated m5S cells. However, enhanced survival was observed only in 3 Gy-irradiated m5S cells with obese ELV treatment. Despite no ELV effect on colony size, interestingly, the frequency of MN in survived m5S cells after 3 Gy irradiation was elevated when treated obese ELVs compared to control ELVs. These results suggested that obese mouse adipose ELVs could enhance the survival of irradiated cells harboring increased radiation-induced genomic instability.</jats:p>

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