A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
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- Geoffrey W Meissner
- Janelia Research Campus, Howard Hughes Medical Institute
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- Aljoscha Nern
- Janelia Research Campus, Howard Hughes Medical Institute
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- Zachary Dorman
- Janelia Research Campus, Howard Hughes Medical Institute
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- Gina M DePasquale
- Janelia Research Campus, Howard Hughes Medical Institute
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- Kaitlyn Forster
- Janelia Research Campus, Howard Hughes Medical Institute
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- Theresa Gibney
- Janelia Research Campus, Howard Hughes Medical Institute
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- Joanna H Hausenfluck
- Janelia Research Campus, Howard Hughes Medical Institute
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- Yisheng He
- Janelia Research Campus, Howard Hughes Medical Institute
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- Nirmala A Iyer
- Janelia Research Campus, Howard Hughes Medical Institute
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- Jennifer Jeter
- Janelia Research Campus, Howard Hughes Medical Institute
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- Lauren Johnson
- Janelia Research Campus, Howard Hughes Medical Institute
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- Rebecca M Johnston
- Janelia Research Campus, Howard Hughes Medical Institute
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- Kelley Lee
- Janelia Research Campus, Howard Hughes Medical Institute
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- Brian Melton
- Janelia Research Campus, Howard Hughes Medical Institute
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- Brianna Yarbrough
- Janelia Research Campus, Howard Hughes Medical Institute
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- Christopher T Zugates
- Janelia Research Campus, Howard Hughes Medical Institute
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- Jody Clements
- Janelia Research Campus, Howard Hughes Medical Institute
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- Cristian Goina
- Janelia Research Campus, Howard Hughes Medical Institute
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- Hideo Otsuna
- Janelia Research Campus, Howard Hughes Medical Institute
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- Konrad Rokicki
- Janelia Research Campus, Howard Hughes Medical Institute
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- Robert R Svirskas
- Janelia Research Campus, Howard Hughes Medical Institute
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- Yoshinori Aso
- Janelia Research Campus, Howard Hughes Medical Institute
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- Gwyneth M Card
- Janelia Research Campus, Howard Hughes Medical Institute
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- Barry J Dickson
- Janelia Research Campus, Howard Hughes Medical Institute
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- Erica Ehrhardt
- Janelia Research Campus, Howard Hughes Medical Institute
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- Jens Goldammer
- Janelia Research Campus, Howard Hughes Medical Institute
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- Masayoshi Ito
- Janelia Research Campus, Howard Hughes Medical Institute
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- Dagmar Kainmueller
- Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC)
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- Wyatt Korff
- Janelia Research Campus, Howard Hughes Medical Institute
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- Lisa Mais
- Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC)
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- Ryo Minegishi
- Janelia Research Campus, Howard Hughes Medical Institute
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- Shigehiro Namiki
- Janelia Research Campus, Howard Hughes Medical Institute
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- Gerald M Rubin
- Janelia Research Campus, Howard Hughes Medical Institute
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- Gabriella R Sterne
- Janelia Research Campus, Howard Hughes Medical Institute
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- Tanya Wolff
- Janelia Research Campus, Howard Hughes Medical Institute
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- Oz Malkesman
- Janelia Research Campus, Howard Hughes Medical Institute
Abstract
<jats:p>Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of <jats:italic>Drosophila</jats:italic> neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here, we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targeting particular neurons. To this end, we have made the images searchable on the NeuronBridge website. We demonstrate the potential of NeuronBridge to rapidly and effectively identify neuron matches based on morphology across imaging modalities and datasets.</jats:p>
Journal
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- eLife
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eLife 12 2023-02-23
eLife Sciences Publications, Ltd
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Details 詳細情報について
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- CRID
- 1360580229821756800
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- ISSN
- 2050084X
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- Data Source
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- Crossref
- KAKEN