The <scp>P4‐ATPase</scp> Drs2 interacts with and stabilizes the multisubunit tethering complex <scp>TRAPPIII</scp> in yeast
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- Irene Pazos
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Marta Puig‐Tintó
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Laura Betancur
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Jorge Cordero
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Nereida Jiménez‐Menéndez
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Marc Abella
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Altair C Hernández
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Ana G Duran
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Emi Adachi‐Fernández
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Carla Belmonte‐Mateos
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Susana Sabido‐Bozo
- Department of Cell Biology University of Seville Seville Spain
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- Sébastien Tosi
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST) Barcelona Spain
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- Akiko Nezu
- Department of Genetics, Graduate School of Medicine Osaka University Osaka Japan
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- Baldomero Oliva
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
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- Julien Colombelli
- Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST) Barcelona Spain
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- Todd R Graham
- Department of Biological Sciences Vanderbilt University Nashville TN USA
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- Tamotsu Yoshimori
- Department of Genetics, Graduate School of Medicine Osaka University Osaka Japan
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- Manuel Muñiz
- Department of Cell Biology University of Seville Seville Spain
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- Maho Hamasaki
- Department of Genetics, Graduate School of Medicine Osaka University Osaka Japan
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- Oriol Gallego
- Department of Medicine and Life Sciences (MELIS) Pompeu Fabra University (UPF) Barcelona Spain
抄録
<jats:title>Abstract</jats:title><jats:p>Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components of the trafficking machinery regulate MTCs through mechanisms that are partially understood. Here, we systematically investigate the interactome that regulates MTCs. We report that P4‐ATPases, a family of lipid flippases, interact with MTCs that participate in the anterograde and retrograde transport at the Golgi, such as TRAPPIII. We use the P4‐ATPase Drs2 as a paradigm to investigate the mechanism and biological relevance of this interplay during transport of Atg9 vesicles. Binding of Trs85, the sole‐specific subunit of TRAPPIII, to the N‐terminal tail of Drs2 stabilizes TRAPPIII on membranes loaded with Atg9 and is required for Atg9 delivery during selective autophagy, a role that is independent of P4‐ATPase canonical functions. This mechanism requires a conserved I(S/R)TTK motif that also mediates the interaction of the P4‐ATPases Dnf1 and Dnf2 with MTCs, suggesting a broader role of P4‐ATPases in MTC regulation.</jats:p>
収録刊行物
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- EMBO reports
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EMBO reports 24 (5), 2023-03-16
Springer Science and Business Media LLC
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詳細情報 詳細情報について
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- CRID
- 1360580230621497728
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- ISSN
- 14693178
- 1469221X
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- データソース種別
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- Crossref
- KAKEN