MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo
-
- Erol C. Bayraktar
- Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, New York, NY 10065;
-
- Lou Baudrier
- Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, New York, NY 10065;
-
- Ceren Özerdem
- Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, New York, NY 10065;
-
- Caroline A. Lewis
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- Sze Ham Chan
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- Tenzin Kunchok
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- Monther Abu-Remaileh
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- Andrew L. Cangelosi
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- David M. Sabatini
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
-
- Kıvanç Birsoy
- Laboratory of Metabolic Regulation and Genetics, The Rockefeller University, New York, NY 10065;
-
- Walter W. Chen
- Whitehead Institute for Biomedical Research, Cambridge, MA 02142;
説明
<jats:title>Significance</jats:title> <jats:p>Mitochondria are intracellular hubs of metabolism that play critical roles in mammals, but knowledge of the metabolic landscape within mitochondria in mammalian tissues remains incomplete. Prior studies have often relied on interrogating metabolites within whole tissue, but this approach does not reveal the behavior of metabolites within specific organelles and cell types. To address this, we generated a transgenic mouse that can express a mitochondrially localized epitope tag (MITO-Tag) with spatiotemporal control to allow for rapid, cell-type-specific immunoisolation of mitochondria from tissues. We demonstrate that these MITO-Tag Mice can be utilized for profiling a variety of molecular components of mitochondria, such as proteins, lipids, and polar metabolites, and thus believe that MITO-Tag Mice will be useful for studying mitochondrial physiology.</jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 116 (1), 303-312, 2018-12-12
Proceedings of the National Academy of Sciences
