<jats:title>Abstract</jats:title><jats:p>Three new species of echinoderid kinorhynchs are described from Daidokutsu, a submarine cave in Ryukyu Islands, Japan. <jats:italic>Echinoderes gama</jats:italic> sp. nov. is characterized by the presence of middorsal acicular spines on segments 4–8; lateroventral acicular spines on segments 7–9; lateroventral tubes on segment 5; sublateral tubes on segment 8; laterodorsal tubes on segment 10; and type-2 gland cell outlets in subdorsal and lateroventral position on segment 2. <jats:italic>Echinoderes kajiharai</jats:italic> sp. nov. is defined by the presence of middorsal acicular spines on segments 4, 6, 8; lateral accessory acicular spines on segment 9; lateroventral acicular spines on segments 6–8; lateroventral tubes on segments 2 and 5; midlateral tubes on segment 10; and type-2 gland cell outlets in laterodorsal position on segments 2 and 5, and subdorsal position on segments 8 and 9. <jats:italic>Echinoderes uozumii</jats:italic> sp. nov. is characterized by the presence of middorsal acicular spines on segments 4 and 6; lateroventral acicular spines on segments 6–9; lateroventral tubes on segments 2 and 5; sublateral tubes on segment 8; laterodorsal tubes on segment 10; type-2 gland cell outlets in subdorsal and lateral accessory position on segment 2; and blunt, short pectinate fringe teeth of primary pectinate fringe on segment 1. In addition, the <jats:italic>Echinoderes multiporus</jats:italic> species group including <jats:italic>E. kajiharai</jats:italic> sp. nov., and the <jats:italic>Echinoderes bispinosus</jats:italic> species group including <jats:italic>E. uozumii</jats:italic> sp. nov. are established. Furthermore, the distribution of the two species groups and the origin of <jats:italic>Echinoderes</jats:italic> species in Daidokutsu are discussed.</jats:p>