CUT&Tag2for1: a modified method for simultaneous profiling of the accessible and silenced regulome in single cells

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Abstract

<jats:title>Abstract</jats:title><jats:p>Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed transposase tethering strategy for in situ chromatin profiling in small samples and single cells. We describe a modified CUT&Tag protocol using a mixture of an antibody to the initiation form of RNA polymerase II (Pol2 Serine-5 phosphate) and an antibody to repressive Polycomb domains (H3K27me3) followed by computational signal deconvolution to produce high-resolution maps of both the active and repressive regulomes in single cells. The ability to seamlessly map active promoters, enhancers, and repressive regulatory elements using a single workflow provides a complete regulome profiling strategy suitable for high-throughput single-cell platforms.</jats:p>

Journal

  • Genome Biology

    Genome Biology 23 (1), 81-, 2022-03-17

    Springer Science and Business Media LLC

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