CCN2 (Cellular Communication Network Factor 2) Deletion Alters Vascular Integrity and Function Predisposing to Aneurysm Formation
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- Raúl R. Rodrigues-Díez
- Molecular and Cellular Biology in Renal and Vascular Pathology, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (R.R.R.-D., A.T.-M., M.O., S.R.-M., M.R.-O.).
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- Antonio Tejera-Muñoz
- Molecular and Cellular Biology in Renal and Vascular Pathology, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (R.R.R.-D., A.T.-M., M.O., S.R.-M., M.R.-O.).
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- Vanesa Esteban
- Department of Allergy and Immunology, FIIS-Fundación Jiménez Díaz, UAM, Asma, Reacciones Adversas y Alérgicas Network. Madrid, Spain (V.E.).
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- Lasse B. Steffensen
- Unit of Cardiovascular and Renal Research, Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark (L.B.S).
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- Raquel Rodrigues-Díez
- Department of Pharmacology, UAM, Instituto de Investigación-Hospital Universitario La Paz, IdiPaz, Ciber Cardiovascular, Madrid, Spain (R.R.-D., A.M.B.).
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- Macarena Orejudo
- Molecular and Cellular Biology in Renal and Vascular Pathology, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (R.R.R.-D., A.T.-M., M.O., S.R.-M., M.R.-O.).
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- Sandra Rayego-Mateos
- Molecular and Cellular Biology in Renal and Vascular Pathology, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (R.R.R.-D., A.T.-M., M.O., S.R.-M., M.R.-O.).
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- Lucas L. Falke
- Department of Pathology, University Medical Center Utrecht, the Netherlands (L.L.F.).
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- Pablo Cannata-Ortiz
- Red de Investigación Renal, Instituto de Salud Carlos III, Madrid, Spain (R.R.R.-D., M.O., S.R.-M., P.C.-O., A.O., M.A.B., M.R.-O.).
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- Alberto Ortiz
- Red de Investigación Renal, Instituto de Salud Carlos III, Madrid, Spain (R.R.R.-D., M.O., S.R.-M., P.C.-O., A.O., M.A.B., M.R.-O.).
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- Jesus Egido
- IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (P.C.-O., A.O., J.E., R.G.).
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- Ziad Mallat
- Department of Medicine, University of Cambridge, United Kingdom (Z.M.).
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- Ana M. Briones
- Department of Pharmacology, UAM, Instituto de Investigación-Hospital Universitario La Paz, IdiPaz, Ciber Cardiovascular, Madrid, Spain (R.R.-D., A.M.B.).
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- M. Auxiliadora Bajo
- Red de Investigación Renal, Instituto de Salud Carlos III, Madrid, Spain (R.R.R.-D., M.O., S.R.-M., P.C.-O., A.O., M.A.B., M.R.-O.).
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- Roel Goldschmeding
- IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (P.C.-O., A.O., J.E., R.G.).
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- Marta Ruiz-Ortega
- Molecular and Cellular Biology in Renal and Vascular Pathology, IIS-Fundación Jiménez Díaz-Universidad Autónoma Madrid, Spain (R.R.R.-D., A.T.-M., M.O., S.R.-M., M.R.-O.).
Description
<jats:sec> <jats:title>Background:</jats:title> <jats:p>CCN2 (cellular communication network factor 2) is a matricellular protein involved in cell communication and microenvironmental signaling responses. CCN2 is known to be overexpressed in several cardiovascular diseases, but its role is not completely understood.</jats:p> </jats:sec> <jats:sec> <jats:title>Methods:</jats:title> <jats:p> Here, CCN2 involvement in aortic wall homeostasis and response to vascular injury was investigated in inducible <jats:italic>Ccn2</jats:italic> -deficient mice, with induction of vascular damage by infusion of Ang II (angiotensin II; 15 days), which is known to upregulate CCN2 expression in the aorta. </jats:p> </jats:sec> <jats:sec> <jats:title>Results:</jats:title> <jats:p> Ang II infusion in CCN2-silenced mice lead to 60% mortality within 10 days due to rapid development and rupture of aortic aneurysms, as evidenced by magnetic resonance imaging, echography, and histological examination. <jats:italic>Ccn2</jats:italic> deletion decreased systolic blood pressure and caused aortic structural and functional changes, including elastin layer disruption, smooth muscle cell alterations, augmented distensibility, and increased metalloproteinase activity, which were aggravated by Ang II administration. Gene ontology analysis of RNA sequencing data identified aldosterone biosynthesis as one of the most enriched terms in CCN2-deficient aortas. Consistently, treatment with the mineralocorticoid receptor antagonist spironolactone before and during Ang II infusion reduced aneurysm formation and mortality, underscoring the importance of the aldosterone pathway in Ang II–induced aorta pathology. </jats:p> </jats:sec> <jats:sec> <jats:title>Conclusions:</jats:title> <jats:p>CCN2 is critically involved in the functional and structural homeostasis of the aorta and in maintenance of its integrity under Ang II–induced stress, at least, in part, by disruption of the aldosterone pathway. Thus, this study opens new avenues to future studies in disorders associated to vascular pathologies.</jats:p> </jats:sec>
Journal
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- Hypertension
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Hypertension 79 (3), 2022-03
Ovid Technologies (Wolters Kluwer Health)
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Details 詳細情報について
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- CRID
- 1360580237002727296
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- ISSN
- 15244563
- 0194911X
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- Data Source
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- Crossref