Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production
説明
<jats:title>Abstract</jats:title><jats:sec><jats:title>Backgrounds</jats:title><jats:p>Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on<jats:italic>Saccharomyces cerevisiae</jats:italic>, achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer<jats:italic>Pichia pastoris</jats:italic>with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate-binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The research promotes the application of<jats:italic>P. pastoris</jats:italic>as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome.</jats:p></jats:sec>
収録刊行物
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- Biotechnology for Biofuels
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Biotechnology for Biofuels 13 (1), 1-, 2020-06-15
Springer Science and Business Media LLC