Reporter–nanobody fusions (RANbodies) as versatile, small, sensitive immunohistochemical reagents
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- Masahito Yamagata
- Center for Brain Science, Harvard University, Cambridge MA, 02138;
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- Joshua R. Sanes
- Center for Brain Science, Harvard University, Cambridge MA, 02138;
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説明
<jats:title>Significance</jats:title><jats:p>Conventional antibodies are often poorly defined, limited in amount, and difficult to store permanently. Nanobodies, recombinant antigen-binding proteins derived from single-chain camelid antibodies, circumvent many of these limitations. To maximize the potential of nanobodies, we fused them to highly sensitive reporters and appended sequences to enable ready production and purification. These RANbodies (reporter and nanobody fusions) can be readily produced in cultured cells, purified in a single step, used to label cells or tissue, and stored indefinitely in the form of DNA or DNA sequences. Given the rapidly increasing rate of nanobody generation, the RANbody platform provides a versatile and scalable platform for immunohistochemical and biochemical analyses.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 115 (9), 2126-2131, 2018-02-13
Proceedings of the National Academy of Sciences