Genomic and Transcriptomic Analysis Identified Novel Putative Cassava lncRNAs Involved in Cold and Drought Stress
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- Rungaroon Suksamran
- Biotechnology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi (Bang KhunThian), Bangkok 10150, Thailand
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- Treenut Saithong
- Bioinformatics and Systems Biology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi (Bang KhunThian), Bangkok 10150, Thailand
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- Chinae Thammarongtham
- Biochemical Engineering and Systems Biology Research Group, National Center for Genetic Engineering and Biotechnology at King Mongkut’s University of Technology Thonburi (Bang KhunThian), Bangkok 10150, Thailand
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- Saowalak Kalapanulak
- Bioinformatics and Systems Biology Program, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi (Bang KhunThian), Bangkok 10150, Thailand
説明
<jats:p>Long non-coding RNAs (lncRNAs) play important roles in the regulation of complex cellular processes, including transcriptional and post-transcriptional regulation of gene expression relevant for development and stress response, among others. Compared to other important crops, there is limited knowledge of cassava lncRNAs and their roles in abiotic stress adaptation. In this study, we performed a genome-wide study of ncRNAs in cassava, integrating genomics- and transcriptomics-based approaches. In total, 56,840 putative ncRNAs were identified, and approximately half the number were verified using expression data or previously known ncRNAs. Among these were 2229 potential novel lncRNA transcripts with unmatched sequences, 250 of which were differentially expressed in cold or drought conditions, relative to controls. We showed that lncRNAs might be involved in post-transcriptional regulation of stress-induced transcription factors (TFs) such as zinc-finger, WRKY, and nuclear factor Y gene families. These findings deepened our knowledge of cassava lncRNAs and shed light on their stress-responsive roles.</jats:p>
収録刊行物
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- Genes
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Genes 11 (4), 366-, 2020-03-28
MDPI AG
