<i>dewA</i> encodes a fungal hydrophobin component of the <i>Aspergillus</i> spore wall
書誌事項
- 公開日
- 1995-04
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1365-2958.1995.tb02389.x
- 公開者
- Wiley
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説明
<jats:title>Summary</jats:title><jats:p>An anonymous cDNA clone, pCAN4, was shown previously to correspond to an mRNA that accumulates preferentially during asexual sporulation of the filamentous fungus <jats:italic>Aspergillus nidulans.</jats:italic> The peptide encoded by pCAN4 is a fungal hydrophobin, a group of small, hydrophobic cell wall proteins. When the CAN4 gene was disrupted, conidia and conidiophores appeared to be normal, but sporulating colonies wetted more rapidly with detergent solutions than did the wild type. We renamed CAN4 <jats:italic>dewA</jats:italic> for the <jats:italic>detergent wettable</jats:italic> phenotype and mapped it to chromosome V, 24 map units from <jats:italic>cysC.</jats:italic> The <jats:italic>A. nidulans rodA</jats:italic> gene also encodes a sporulation‐specific fungal hydrophobin. Spores of a <jats:italic>dewA rodA</jats:italic> double mutant were less hydrophobic than those of either mutant alone, showing that <jats:italic>dewA</jats:italic> and <jats:italic>rodA</jats:italic> contribute independently to spore‐wall hydrophobicity, Immuno‐localization of DewA by epitope tagging demonstrated that DewA is present in the spore wall, but not in the walls of germ tubes, hyphae or cells of the spore‐producing conidiophore. We conclude that <jats:italic>dewA</jats:italic> encodes a new fungal hydrophobin component of the conidial wall.</jats:p>
収録刊行物
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- Molecular Microbiology
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Molecular Microbiology 16 (1), 33-44, 1995-04
Wiley