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Ultrasound stimulation induces microRNA expression changes that could be involved in sonication-induced apoptosis
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Description
The purpose of this study is to investigate the involvement of microRNAs (miRNAs) in sonication-induced apoptosis.U937 cells derived from human leukemia were sonicated with 1-MHz ultrasound at 0.4 W/cm(2) and 10 % duty factor for 60 s, a condition inducing apoptosis. The total RNA was extracted from cells at various timings after sonication and subjected to microarray and real-time PCR for miRNA expression analyses.Expression of several miRNAs was significantly affected by sonication. For miR-424* and miR-720, whose expressions were eminently decreased by sonication, cell lines overexpressing these miRNAs were established. Conversely, for miR-663B and miR-663, whose expressions were eminently increased by sonication, cell lines inhibiting these miRNA functions were established. When these cell lines were sonicated, a cell line inhibiting miR-663B function significantly increased sonication-induced apoptosis, suggesting this may be involved in cellular responses to sonication. Two genes that could induce apoptosis, KSR2 and CREBZF, were identified as potential target genes of miR-663B since potential target sequences on their 3' UTR mediated to decrease expression of a reporter gene.These results suggest that miRNAs may be involved in cellular responses to ultrasound through their expression changes caused by sonication.
Journal
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- Journal of Medical Ultrasonics
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Journal of Medical Ultrasonics 39 (4), 207-216, 2012-05-03
Springer Science and Business Media LLC
