Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis
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- Hirofumi Harada
- Department of Applied Chemistry
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- Akira Onoda
- Department of Applied Chemistry
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- Takayuki Uchihashi
- Department of Physics
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- Hiroki Watanabe
- Faculty of Natural Science and Technology
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- Naoki Sunagawa
- Department of Biomaterials Sciences
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- Masahiro Samejima
- Department of Biomaterials Sciences
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- Kiyohiko Igarashi
- Department of Biomaterials Sciences
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- Takashi Hayashi
- Department of Applied Chemistry
Abstract
<p>To visualize the dynamic domain motion of class-I CDH from <italic>Phanerochaete chrysosporium</italic> (<italic>Pc</italic>CDH) during catalysis using high-speed atomic force microscopy, the apo-form of <italic>Pc</italic>CDH was anchored to a heme-immobilized flat gold surface that can fix the orientation of the CYT domain.</p>
Journal
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- Chemical Science
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Chemical Science 8 (9), 6561-6565, 2017
Royal Society of Chemistry (RSC)
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Details 詳細情報について
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- CRID
- 1360846641832175488
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- ISSN
- 20416539
- 20416520
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- Data Source
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- Crossref
- KAKEN