VASCULAR‐RELATED NAC‐DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation
書誌事項
- 公開日
- 2011-03-07
- 資源種別
- journal article
- 権利情報
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- http://onlinelibrary.wiley.com/termsAndConditions#vor
- DOI
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- 10.1111/j.1365-313x.2011.04514.x
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Summary</jats:title><jats:p>The <jats:italic>Arabidopsis thaliana</jats:italic> NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), acts as a key regulator of xylem vessel differentiation. In order to identify direct target genes of VND7, we performed global transcriptome analysis using Arabidopsis transgenic lines in which VND7 activity could be induced post‐translationally. This analysis identified 63 putative direct target genes of VND7, which encode a broad range of proteins, such as transcription factors, IRREGULAR XYLEM proteins and proteolytic enzymes, known to be closely associated with xylem vessel formation. Recombinant VND7 protein binds to several promoter sequences present in candidate direct target genes: specifically, in the promoter of <jats:italic>XYLEM CYSTEINE PEPTIDASE1</jats:italic>, two distinct regions were demonstrated to be responsible for VND7 binding. We also found that expression of <jats:italic>VND7</jats:italic> restores secondary cell wall formation in the fiber cells of inflorescence stems of <jats:italic>nst1</jats:italic> <jats:italic>nst3</jats:italic> double mutants, as well as expression of <jats:italic>NAC SECONDARY WALL THICKENING PROMOTING FACTOR3</jats:italic> (<jats:italic>NST3</jats:italic>, however, the vessel‐type secondary wall deposition was observed only as a result of <jats:italic>VND7</jats:italic> expression. These findings indicated that VND7 upregulates, directly and/or indirectly, many genes involved in a wide range of processes in xylem vessel differentiation, and that its target genes are partially different from those of NSTs.</jats:p>
収録刊行物
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- The Plant Journal
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The Plant Journal 66 (4), 579-590, 2011-03-07
Wiley
