SymB and SymC, two membrane associated proteins, are required for <scp><i>E</i></scp><i>pichloë festucae</i> hyphal cell–cell fusion and maintenance of a mutualistic interaction with <scp><i>L</i></scp><i>olium perenne</i>
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- Kimberly A. Green
- Institute of Fundamental Sciences, Massey University Palmerston North 4442 New Zealand
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- Yvonne Becker
- Institute of Fundamental Sciences, Massey University Palmerston North 4442 New Zealand
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- Aiko Tanaka
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya 464‐8601 Japan
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- Daigo Takemoto
- Graduate School of Bioagricultural Sciences Nagoya University Nagoya 464‐8601 Japan
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- Helen L. Fitzsimons
- Institute of Fundamental Sciences, Massey University Palmerston North 4442 New Zealand
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- Stephan Seiler
- Freiburg Institute for Advanced Studies, Albert‐Ludwigs Universität Freiburg Freiburg Germany
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- Hervé Lalucque
- Université Paris Diderot, Sorbonne Paris Cité, Laboratoire des Energies de Demain Paris 75205 France
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- Philippe Silar
- Université Paris Diderot, Sorbonne Paris Cité, Laboratoire des Energies de Demain Paris 75205 France
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- Barry Scott
- Institute of Fundamental Sciences, Massey University Palmerston North 4442 New Zealand
書誌事項
- 公開日
- 2016-12-14
- 資源種別
- journal article
- 権利情報
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- http://creativecommons.org/licenses/by-nc-nd/4.0/
- DOI
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- 10.1111/mmi.13580
- 公開者
- Wiley
この論文をさがす
説明
<jats:title>Summary</jats:title><jats:p>Cell–cell fusion in fungi is required for colony formation, nutrient transfer and signal transduction. Disruption of genes required for hyphal fusion in <jats:italic>Epichloë festucae</jats:italic>, a mutualistic symbiont of <jats:italic>Lolium</jats:italic> grasses, severely disrupts the host interaction phenotype. They examined whether <jats:italic>symB</jats:italic> and <jats:italic>symC</jats:italic>, the <jats:italic>E. festucae</jats:italic> homologs of <jats:italic>Podospora anserina</jats:italic> self‐signaling genes <jats:italic>IDC2</jats:italic> and <jats:italic>IDC3</jats:italic>, are required for <jats:italic>E. festucae</jats:italic> hyphal fusion and host symbiosis. Deletion mutants of these genes were defective in hyphal cell fusion, formed intra‐hyphal hyphae, and had enhanced conidiation. SymB‐GFP and SymC‐mRFP1 localize to plasma membrane, septa and points of hyphal cell fusion. Plants infected with Δ<jats:italic>symB</jats:italic> and Δ<jats:italic>symC</jats:italic> strains were severely stunted. Hyphae of the mutants colonized vascular bundles, were more abundant than wild type in the intercellular spaces and formed intra‐hyphal hyphae. Although these phenotypes are identical to those previously observed for cell wall integrity MAP kinase mutants no difference was observed in the basal level of MpkA phosphorylation or its cellular localization in the mutant backgrounds. Both genes contain binding sites for the transcription factor ProA. Collectively these results show that SymB and SymC are key components of a conserved signaling network for <jats:italic>E. festucae</jats:italic> to maintain a mutualistic symbiotic interaction within <jats:italic>L. perenne</jats:italic>.</jats:p>
収録刊行物
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- Molecular Microbiology
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Molecular Microbiology 103 (4), 657-677, 2016-12-14
Wiley
