Roles of Epithelial Cell–Derived Type 2–Initiating Cytokines in Experimental Allergic Conjunctivitis

DOI Web Site PubMed 被引用文献10件 オープンアクセス
  • Yosuke Asada
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan 2Frontier Research Initiative, Institute of Medical Science, University of Tokyo, Tokyo, Japan
  • Susumu Nakae
    Frontier Research Initiative, Institute of Medical Science, University of Tokyo, Tokyo, Japan
  • Waka Ishida
    Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan
  • Kanji Hori
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Jobu Sugita
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Katsuko Sudo
    Animal Research Center, Tokyo Medical University, Tokyo, Japan
  • Ken Fukuda
    Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan
  • Atsuki Fukushima
    Department of Ophthalmology, Kochi University School of Medicine, Nangoku, Japan
  • Hajime Suto
    Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan 6Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan
  • Akira Murakami
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Hirohisa Saito
    Department of Allergy and Immunology, National Research Institute for Child Health and Development, Tokyo, Japan
  • Nobuyuki Ebihara
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Akira Matsuda
    Laboratory of Ocular Atopic Diseases Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan

説明

To clarify the possible involvement of the type 2-initiating cytokines interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP) in the pathophysiology of allergic conjunctivitis, we evaluated ragweed (RW)-induced experimental allergic conjunctivitis (EAC) models by using IL-25 knockout (KO), IL-33 KO, and TSLP receptor (TSLPR) KO mice.Interleukin-25 KO, IL-33 KO, TSLPR KO, and BALB/c wild-type mice were sensitized twice with RW in alum and then challenged with RW in eye drops. Clinical scores and eosinophil infiltration were evaluated. Expression levels of serum immunoglobulin E (IgE) and cytokines in the conjunctival tissues were quantified and immunohistochemical analysis was carried out.Significant reductions in clinical scores and numbers of infiltrating eosinophils were observed in the RW-EAC model using IL-33 KO mice. There were no significant differences in clinical scores and numbers of infiltrating eosinophils among IL-25KO, TSLPR KO, and wild-type mice. Serum IgE concentration was upregulated after RW challenges, and there were no differences among the mouse genotypes. Expression levels of of il4, il5, il13, and ccl5 mRNA were diminished in the conjunctivae of the RW-EAC model using IL-33 KO mice compared to those in wild-type mice. Interleukin-33 expression was upregulated as early as 1 hour after RW eye-drop challenge. The number of infiltrating basophils in the conjunctivae of the RW-EAC model using IL-33 KO mice was diminished compared to that in wild-type mice.Among the type 2-initiating cytokines, IL-33 may play a major role in conjunctival inflammation in an RW-EAC model.

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