A critical time window of<i>Sry</i>action in gonadal sex determination in mice

DOI PDF PubMed 被引用文献28件 参考文献42件 オープンアクセス
  • Ryuji Hiramatsu
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1,Bunkyoku, Tokyo 113-8657, Japan.
  • Shogo Matoba
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1,Bunkyoku, Tokyo 113-8657, Japan.
  • Masami Kanai-Azuma
    Department of Anatomy, Kyorin University School of Medicine, Shinkawa 6-20-2,Mitaka, Tokyo 181-8611, Japan.
  • Naoki Tsunekawa
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1,Bunkyoku, Tokyo 113-8657, Japan.
  • Yuko Katoh-Fukui
    Department of Aging Intervention, National Institute for Longevity Sciences,National Center for Geriatrics and Gerontology, Gengo 36-3, Morioka-cho, Obu,Aichi 474-8511, Japan.
  • Masamichi Kurohmaru
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1,Bunkyoku, Tokyo 113-8657, Japan.
  • Ken-ichirou Morohashi
    Department of Molecular Biology, Graduate School of Medicine, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
  • Dagmar Wilhelm
    Institute for Molecular Bioscience, The University of Queensland, St Lucia,Brisbane, QLD 4072, Australia.
  • Peter Koopman
    Institute for Molecular Bioscience, The University of Queensland, St Lucia,Brisbane, QLD 4072, Australia.
  • Yoshiakira Kanai
    Department of Veterinary Anatomy, The University of Tokyo, Yayoi 1-1-1,Bunkyoku, Tokyo 113-8657, Japan.

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<jats:p>In mammals, the Y-linked sex-determining gene Srycell-autonomously promotes Sertoli cell differentiation from bipotential supporting cell precursors through SRY-box containing gene 9 (Sox9),leading to testis formation. Without Sry action, the supporting cells differentiate into granulosa cells, resulting in ovarian development. However,how Sry acts spatiotemporally to switch supporting cells from the female to the male pathway is poorly understood. We created a novel transgenic mouse line bearing an inducible Sry transgene under the control of the Hsp70.3 promoter. Analysis of these mice demonstrated that the ability of Sry to induce testis development is limited to approximately 11.0-11.25 dpc, corresponding to a time window of only 6 hours after the normal onset of Sry expression in XY gonads. If Sry was activated after 11.3 dpc, Sox9 activation was not maintained, resulting in ovarian development. This time window is delimited by the ability to engage the high-FGF9/low-WNT4 signaling states required for Sertoli cell establishment and cord organization. Our results indicate the overarching importance of Sry action in the initial 6-hour phase for the female-to-male switching of FGF9/WNT4 signaling patterns.</jats:p>

収録刊行物

  • Development

    Development 136 (1), 129-138, 2009-01-01

    The Company of Biologists

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