Roles of Mono- and Divalent Cations in DNA Compaction Induced by Histone-Mimic Nanoparticles
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- Toshio Takenaka
- Department of Material Chemistry, Graduate School of Environmental Studies, Nagoya University
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- Anatoly A Zinchenko
- Department of Material Chemistry, Graduate School of Environmental Studies, Nagoya University
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- Kenichi Yoshikawa
- Spatio-Temporal Order Project, ICORP, JST
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- Shizuaki Murata
- Department of Material Chemistry, Graduate School of Environmental Studies, Nagoya University
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<jats:title>Abstract</jats:title> <jats:p>The compaction of gene-size T4 DNA induced by the action of silica nanoparticles (10–100 nm diameter), the surfaces of which were modified to various degrees by a 3-aminopropylsilyl groups, was monitored by a fluorescent microscope in an aqueous solution. It was found that DNA compaction was facilitated by mono- and divalent cations such as Na+, K+, Mg2+, and Ca2+. This promotion by cations was the result of charge-neutralization of DNA and was more remarkable in compaction by histone-size nanoparticles (10 nm) than in that by larger ones (>15 nm). Divalent metal ions were more effective at promoting compaction due to their greater charge-neutralizing character, but a quaternary diammonium derivative of 1,3-propanediamine deactivated DNA compaction by 10-nm nanoparticles. Although metal ions binding to DNA can reduce electrostatic tension on DNA and provide flexibility to create a DNA loop with a small diameter, such ammonium dication does not make DNA sufficiently flexible.</jats:p>
収録刊行物
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- Bulletin of the Chemical Society of Japan
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Bulletin of the Chemical Society of Japan 83 (6), 643-650, 2010-05-28
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1360846644026892928
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- NII論文ID
- 130004152738
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- ISSN
- 13480634
- 00092673
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