Double‐Strand Breaks in Genome‐Sized DNA Caused by Ultrasound
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- Rinko Kubota
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
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- Yusuke Yamashita
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
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- Takahiro Kenmotsu
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
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- Yuko Yoshikawa
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
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- Kenji Yoshida
- Center for Frontier Medical Engineering Chiba University Chiba 263-0022 Japan
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- Yoshiaki Watanabe
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
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- Tadayuki Imanaka
- Research Organization of Science and Technology Ritsumeikan University Kusatsu 525–8577 Japan
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- Kenichi Yoshikawa
- Faculty of Life and Medical Sciences Doshisha University Tatara-Miyakodani, Kyotanabe Kyoto 610-0321 Japan
説明
<jats:title>Abstract</jats:title><jats:p>DNA double‐strand breaks (DSBs) caused by ultrasound were evaluated in a quantitative manner by single‐molecule fluorescence microscopy. We compared the effect of time‐interval (or pulse) sonication to that of continuous wave (CW) sonication at a fixed frequency of 30 kHz. Pulses caused fewer DSBs than CW sonication under the same total input ultrasound energy when the pulse repetition period was above the order of a second. In contrast, pulses caused more DSBs than CW sonication for pulse widths shorter than a second. These effect of ultrasound on DNA were interpreted in terms of the time‐dependent decay in the probability of breakage during the duration of a pulse. We propose a simple phenomenological model by considering a characteristic decay in the probability of DSBs during single‐pulse sonication, which reproduces the essence of the experimental trend. In addition, a data analysis revealed a characteristic scaling behavior between the number of pulses and the number of DSBs.</jats:p>
収録刊行物
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- ChemPhysChem
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ChemPhysChem 18 (8), 959-964, 2017-03-14
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360848655007028096
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- ISSN
- 14397641
- 14394235
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- データソース種別
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- Crossref
- KAKEN