HPLC/UV Analysis of Polyacetylenes, Phenylpropanoid and Pyrrolidine Alkaloids in Medicinally Used<i>Codonopsis</i>Species

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  • Jing-Yu He
    Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine; University of Toyama; 2630 Sugitani Toyama 930-0194 Japan
  • Shu Zhu
    Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine; University of Toyama; 2630 Sugitani Toyama 930-0194 Japan
  • Katsuko Komatsu
    Division of Pharmacognosy, Department of Medicinal Resources, Institute of Natural Medicine; University of Toyama; 2630 Sugitani Toyama 930-0194 Japan

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  • HPLC/UV Method for Quality Analysis of Codonopsis Radix

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Codonopsis Radix is commonly used as a tonic in traditional Chinese medicine. However, there is no suitable method to assess the quality of Codonopsis Radix based on multiple components having potential bioactivities.To establish a HPLC/UV method for simultaneous quantitation of polyacetylenes (lobetyol, lobetyolin, lobetyolinin, cordifolioidyne B), phenylpropanoid (tangshenoside I) and pyrrolidine alkaloids (codonopyrrolidiums A, B) in Codonopsis Radix.Large-scale methanol extraction of Codonopsis Radix, followed by chromatographic separation, provided the seven analytes for quantitation standards. Ultrasound-assisted methanol extracts of samples were analysed using reversed phase, gradient elution HPLC monitored at 215 nm.The method developed allowed efficient separation of the seven compounds and the detection and quantitation limits of the seven analytes were 0.10-0.32 µg/mL and 0.35-1.07 µg/mL, respectively. All calibration curves showed good linearities (r0.9993) within the test ranges. Intraday and interday precisions were good with RSD2.84%. The recoveries of all analytes ranged from 95.8 to 104.7%.HPLC/UV is an efficient and accurate method of analysis for simultaneous quantitation of seven components in Codonopsis Radix.

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