Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice
書誌事項
- 公開日
- 2012-10-20
- 資源種別
- journal article
- 権利情報
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- https://www.springernature.com/gp/researchers/text-and-data-mining
- https://www.springernature.com/gp/researchers/text-and-data-mining
- DOI
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- 10.1007/978-1-62703-227-8_18
- 公開者
- Humana Press
この論文をさがす
説明
Clarification of the spatiotemporal regulation and function of immune cells within the skin is critical to the understanding of the role of immune cells and the skin in immune homeostasis. Here, we describe a novel assay system for monitoring cell movements in the entire body using the photoconvertible fluorescent protein "Kaede"-transgenic (Tg) mice. We can label immune cells by the change in color of Kaede from green to red in these cells following exposure to violet light and track these cells in the entire body. The Kaede-Tg system is an ideal tool for monitoring precise cellular movements between the skin and other anatomical sites in vivo at different stages of the immune response.
収録刊行物
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- Methods in Molecular Biology
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Methods in Molecular Biology 961 279-286, 2012-10-20
Humana Press