Quantitative Analysis of Coenzyme F430 in Environmental Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane Oxidation
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- Masanori Kaneko
- Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan
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- Yoshinori Takano
- Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan
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- Yoshito Chikaraishi
- Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan
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- Nanako O. Ogawa
- Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan
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- Susumu Asakawa
- Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
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- Takeshi Watanabe
- Laboratory of Soil Biology and Chemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan
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- Seigo Shima
- Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Strasse 10, D-35043 Marburg, Germany
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- Martin Krüger
- Federal Institute for Geosciences and Natural Resources, Stilleweg 2, D-30655 Hannover, Germany
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- Makoto Matsushita
- Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka 422-8529, Japan
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- Hiroyuki Kimura
- Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka 422-8529, Japan
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- Naohiko Ohkouchi
- Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka 237-0061, Japan
書誌事項
- 公開日
- 2014-03-21
- 資源種別
- journal article
- 権利情報
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- http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html
- DOI
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- 10.1021/ac500305j
- 公開者
- American Chemical Society (ACS)
この論文をさがす
説明
Coenzyme F430 is a nickel hydrocorphinoid and is the prosthetic group of methyl-coenzyme M reductase that catalyzes the last step of the methanogenic reaction sequence and its reversed reaction for anaerobic methane oxidation by ANME. As such, function-specific compound analysis has the potential to reveal the microbial distribution and activity associated with methane production and consumption in natural environments and, in particular, in deep subsurface sediments where microbiological and geochemical techniques are restricted. Herein, we report the development of a technique for high-sensitivity analysis of F430 in environmental samples, including paddy soils, marine sediments, microbial mats, and an anaerobic groundwater. The lower detection limit of F430 analysis by liquid chromatography/mass spectrometry is 0.1 femto mol, which corresponds to 6 × 10(2) to 1 × 10(4) cells of methanogens. F430 concentrations in these natural environmental samples range from 63 × 10(-6) to 44 nmol g(-1) and are consistent with the methanogenic archaeal biomass estimated by microbiological analyses.
収録刊行物
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- Analytical Chemistry
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Analytical Chemistry 86 (7), 3633-3638, 2014-03-21
American Chemical Society (ACS)
