Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells
説明
<jats:title>Abstract</jats:title><jats:p>It remains unclear how readiness for Ca<jats:sup>2+</jats:sup>-dependent exocytosis depends on varying degrees of SNARE complex assembly. Here we directly investigate the SNARE assembly using two-photon fluorescence lifetime imaging (FLIM) of Förster resonance energy transfer (FRET) between three pairs of neuronal SNAREs in presynaptic boutons and pancreatic β cells in the islets of Langerhans. These FRET probes functionally rescue their endogenous counterparts, supporting ultrafast exocytosis. We show that<jats:italic>trans</jats:italic>-SNARE complexes accumulated in the active zone, and estimate the number of complexes associated with each docked vesicle. In contrast, SNAREs were unassembled in resting state, and assembled only shortly prior to insulin exocytosis, which proceeds slowly. We thus demonstrate that distinct states of fusion readiness are associated with SNARE complex formation. Our FRET/FLIM approaches enable optical imaging of fusion readiness in both live and chemically fixed tissues.</jats:p>
収録刊行物
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- Nature Communications
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Nature Communications 6 (1), 8531-, 2015-10-06
Springer Science and Business Media LLC
